Antioxidant phenylpropanoid glycosides from Buddleja davidii

Figures & data

Figure 1.  Structures of Compounds 1–10.

Table 1.  Antioxidative activity of the solvent fractions.

Sample	IC50 [μg/mL]^a
	•OH^b	Total ROS^c	ONOO^−d
CHCl3	51.42 ±  0.05	>400	56.41 ±  0.09
EtOAc	27.17 ±  0.03	>300	31.09 ±  0.02
n-BuOH	7.39 ±  0.04	49.21 ±  0.07	9.10 ±  0.05
H2O	62.25 ±  0.06	>400	69.29 ±  0.01
Trolox ^e	5.50 ± 0.07	35.07 ± 0.09	–
DL-Penicillamine^f	–	–	1.03 ± 0.05

^a Values of •OH, total ROS, and ONOO⁻ are expressed as the mean ± standard error of triplicate experiments.

^b Inhibitory activity of hydroxyl radical generation in 1.0 mM H₂O₂ and 0.2 mM FeSO₄.

^c Inhibitory activity of total ROS generation in kidney postmicrosomal fraction.

^d Inhibitory activity of authentic peroxynitrite.

^e Trolox was used as a positive control.

^f DL-Penicillamine was used as a positive control.

Table 2.  Antioxidative activity of Compounds 1–10.

Compound	IC50 [μg/mL]^a
	•OH^b	Total ROS^c	ONOO^−d
1	4.15 ±  0.07	40.32 ±  0.09	2.26 ±  0.03
2	8.10 ±  0.01	73.12 ± 0.02	5.31 ±  0.06
3	5.40 ±  0.04	47.51 ±  0.07	3.09 ±  0.09
4	5.09 ±  0.09	43.10 ±  0.06	2.69 ±  0.07
5	6.85 ±  0.02	62.29 ±  0.09	4.01 ±  0.03
6	5.90 ±  0.05	54.34 ±  0.04	3.18 ±  0.09
7	4.72 ±  0.01	43.71 ±  0.09	2.71 ±  0.07
8	9.47 ±  0.03	81.15 ± 0.07	7.79 ±  0.04
9	7.30 ±  0.07	69.93 ±  0.05	5.13 ±  0.06
10	7.54 ±  0.06	71.19 ±  0.08	5.29 ±  0.01
Trolox^e	2.79 ± 0.05	30.09 ± 0.10	–
DL-Penicillamine^f	–	–	1.03 ± 0.03

^a Values of •OH, total ROS, and ONOO⁻ are expressed as the mean ± standard error of triplicate experiments.

^b Inhibitory activity of hydroxyl radical generation in 1.0 mM H₂O₂ and 0.2 mM FeSO₄.

^c Inhibitory activity of total ROS generation in kidney postmicrosomal fraction.

^d Inhibitory activity of authentic peroxynitrite.

^e Trolox was used as a positive control.

^f DL-Penicillamine was used as a positive control.