Expression and correlation analysis of silent information regulator 1 (SIRT1), sterol regulatory element-binding protein-1 (SREBP1), and pyroptosis factor in gestational diabetes mellitus

Figures & data

Table 1. Primer sequences.

	Primer sets
SIRT1	Forward 5′-CAAAGGAGCAGATTAGTAGGCG-3′
	Reverse 5′-CTCTGGCATGTCCCACTATCAC-3′
SREBP1	Forward 5′-GCCTATTTAACCCACCCTATG-3′
	Reverse 5′-TGGCACTGACTCTTCCTTGAT-3′
GAPDH	Forward 5′-GGAGTCCACTGGCGTCTTCA-3′
	Reverse 5′-GTCATGAGTCCTTCCACGATACC-3′

Table 2. Clinical materials and laboratory characteristics of the participant.

Characteristics	Control (n = 50)	GDM (n = 50)	p-Value
Age (years)	30.82 ± 3.53	31.9 ± 3.16	0.110
Pre-pregnancy BMI (kg/m^2)	21.92 ± 2.30	23.18 ± 3.50	0.037
Gestational weight gain (kg)	11.74 ± 4.5	11.84 ± 4.67	0.914
Gestational week of labor	39.57 ± 0.87	38.75 ± 1.11	<0.0001
FPG (mmol/L)	4.54 ± 0.39	4.96 ± 0.41	<0.0001
OGTT-1h (mmol/L)	7.16 ± 1.32	9.62 ± 1.32	<0.0001
OGTT-2h (mmol/L)	6.77 ± 1.04	8.54 ± 0.98	<0.0001
FINS	10.34 ± 1.78	11.80 ± 1.83	0.0001
HOMA-IR	2.08 ± 0.38	2.61 ± 0.49	<0.0001
HbA1c (%)	5.10 ± 0.25	5.44 ± 0.32	<0.0001
Neonatal weight (kg)	3.24 ± 0.23	3.37 ± 0.33	0.028
Placenta mass (g)	610.3 ± 52.50	630.6 ± 72.91	0.114

FPG: fasting plasma glucose; OGTT-1h: 1-h oral glucose tolerance test; OGTT-2h: 2-h oral glucose tolerance test; FINS: fasting insulin; HOMA-IR: homeostatic model assessment of insulin resistance; HbA1c: insulin, glycated hemoglobin A1c.

Data are presented as mean ± standard deviation.

Table 3. The results of ELISA for SIRT1, SREBP1, and pyroptosis factor in two groups.

	Control (n = 50)	GDM (n = 50)	p-Value
SIRT1 (ng/L)	344.8 ± 175.3	238.8 ± 125.2	0.0008
SREBP1 (ng/L)	1905 ± 1121	2546 ± 1174	0.006
NLRP3 (ng/L)	492.7 ± 114.6	633.2 ± 106.7	<0.0001
Caspase-1 (ng/L)	27.36 ± 11.88	45.71 ± 12.46	<0.0001
IL-1β (ng/L)	34.55 ± 9.31	45.36 ± 8.42	<0.0001
IL-18 (ng/L)	140.5 ± 43.15	209.7 ± 35.71	<0.0001

Data are presented as mean ± standard deviation.

Figure 1. Relative mRNA expression of SIRT1 and SREBP1 in control and GDM groups.

Figure 2. Relative protein expression of SIRT1 and SREBP1 in control and GDM groups. (A) Western blot detection of SIRT1 protein expression levels in placental tissues. (B) Western blot detection of SREBP1 protein expression levels in placental tissues. (C) SIRT1 and SREBP1 were significantly differentially expressed in the placental tissues of the two groups.

Figure 3. Correlations of SIRT1 with biochemical parameters and pyroptosis factors.

Figure 4. Correlations of SREBP1 with pyroptosis factors.

Data availability statement

The data that support the findings of this study are available from the corresponding author, Ning Han, upon reasonable request.