Figures & data
FIGURE 1. Chemical structure of triphlorethol-A.
FIGURE 2. Effect of triphlorethol-A on HCHO-induced DNA strand breaks. (A) The cell lysates were electrophoresed and the phosphohistone H2A.X was detected using a specific antibody. (B) Representative images and % cellular DNA damage detected by comet assay. Asterisk indicates significantly different from control (p < .05); **significantly different from HCHO-treated cells (p < .05). (C) Ku 70 and phospho DNA-PKcs proteins were detected using specific antibodies (color figure available online).
FIGURE 3. Effect of triphlorethol-A on HCHO-induced 8-OHdG base modification. (A) The amount of 8-OHdG in DNA was determined using the Bioxytech 8-OHdG-ELISA kit. Asterisk indicates significantly different from control (p < .05); **significantly different from HCHO- treated cells (p < .05). (B) 8-OHdG levels reflected by the binding of avidin-TRITC were visualized with a fluorescence microscope. (C) OGG1 protein was detected using a specific antibody (color figure available online).
FIGURE 4. Effect of triphlorethol-A on the the phosphorylation of Akt and cell viability. (A) Cell lysates were electrophoresed, and phospho Akt was detected by a specific antibody. (B) After treatment with LY294002 for 1 h, followed by treatment with triphlorethol-A for 1 h, and finally 150 μM of HCHO for 24 h, cell viability was assessed using the MTT assay. Asterisk indicates significantly different from control (p < .05); **significantly different from HCHO-treated cells (p < .05); ***significantly different from triphlorethol-A plus HCHO-treated cells (p < .05).
