Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries

Figures & data

Table 1 The gradient solvent mixture execution.

Time (min)	Methanol (%)	0.5 mM TEAP buffer pH 5.6 (%)	H2O (%)	Acetonitrile (%)
0	70	11	0	19
22	70	11	0	19
24	70	4	0	26
30	69	0	5	26
40	0	0	5	95
45	70	11	0	19

0.5 mM TEAP buffer pH 5.6: 0.5 mM phosphoric acid, pH brought to point with triethylamine.

Table 2 Separated and determined fatty acids by the method described above

Carbon atom number and double bond number	Fatty acid
Saturated fatty acids (SAFAs)
C 12:0	lauric
C 14:0	myristic
C 16:0	palmitic
C 18:0	stearic
C 20:0	arachidic
C 22:0	behenic
Monounsaturated fatty acids (MUFAs)
C 14:1	myristoleic
C 16:1	palmitoleic
C 18:1	oleic
C 24:1	nervonic
Polyunsaturated fatty acids (PUFAs)
C 18:2	linoleic
C 18:3	linolenic
C 20:4	arachidonic
C 20:5	eicosapentaenoic
C 22:6	docosahexaenoic

Table 3 Fatty acid content in cholesteryl esters of umbilical cord arteries

	Control UCA	Preeclamptic
Fatty acid	wall [mol%]	UCA wall
SAFA
C 12:0	5.05 ± 0.82	15.52 ± 0.99
C 14:0	3.09 ± 0.41	0.89 ± 0.29
C 16:0	13.14 ± 1.03	5.73 ± 0.94
C 18:0	24.90 ± 2.05	12.00 ± 1.98
C 20:0	1.13 ± 0.17	6.71 ± 0.21
C 22:0	n.d.	1.62 ± 0.19
MUFA
C 14:1	5.47 ± 0.22	1.45 ±0.17
C 16:1	2.62 ± 0.18	n.d.
C 18:1	23.91 ± 1.96	9.75 ± 1.78
C 24:1	n.d.	1.62 ± 0.17
PUFA
C 18:2	6.02 ± 0.59	4.11 ± 0.51
C 18:3	1.05 ± 0.20	7.35 ± 0.29
C 20:4	4.91 ± 0.31	1.77 ± 0.25
C 20:5	5.03 ± 0.77	13.21 ± 0.82
C 22:6	3.70 ± 0.52	18.28 ± 0.61

n.d., not detected.

Table 4 Percentage of saturated (SAFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids from cholesteryl esters of umbilical cord arteries

	Control UCA wall	Preeclamptic UCA wall
SAFA	47.31	42.47
MUFA	32.00	12.82
PUFA	20.71	44.72