Oxidative Modifications of Rat Liver Cell Components During Fasciola hepatica Infection

Figures & data

Table 1 TAS in the liver of control and F. hepatica-infected rats at 4, 7, and 10 wpi

	Weeks postinfection
	4		7		10
	Control rats	Infected rats	Control rats	Infected rats	Control rats	Infected rats
TAS mol/g tissue	110.6 ± 6.5	93.5 ± 7.3^a	108.2 ± 6.9	91.4 ± 7.5^a	112.5 ± 6.1	98.8 ± 7.7^a

^a Significantly different from control group (p <0.05).

Table 2 The levels of lipid peroxidation products: conjugated dienes (CDs) lipid hydroperoxides (LOOHs), malondialdehyde (MDA), and 4-hydroxynonenal (4-HNE) in the liver of control and F. hepatica-infected rats at 4, 7, and 10 wpi

	Weeks postinfection
	4		7		10
	Control rats	Infected rats	Control rats	Infected rats	Control rats	Infected rats
CD (μmol/g tissue)	1.27 ± 0.05	1.34 ± 0.12	1.30 ± 0.06	1.39 ± 0.14	1.29 ± 0.06	1.48 ± 0.14
LOOH (μmol/g tissue)	124 ± 8	187 ± 14	122 ± 8	181 ± 15	127 ± 9	167 ± 14
MDA (nmol/g tissue)	2.58 ± 0.15	5.09 ± 0.52^a	2.74 ± 0.15	4.79 ± 0.52^a	2.71 ± 0.15	4.58 ± 0.52^a
4-HNE (nmol/g tissue)	1.36 ± 0.08	2.84 ± 0.29^a	1.27 ± 0.08	2.53 ± 0.23^a	1.32 ± 0.23^a	2.28 ± 0.23^a

^aSignificantly different from control group (p < 0.05).

Table 3 The levels of protein modification markers: carbonyl groups, dityrosine, tryptophan, sulfhydryl groups, and amino groups in the liver of control and F. hepatica-infected rats at 4, 7, and 10 wpi

	Weeks postinfection
	4		7		10
	Control rats	Infected rats	Control rats	Infected rats	Control rats	Infected rats
Carbonyl groups (nmol/mg protein)	0.97 ± 0.05	1.17 ± 0.08^a	0.99 ± 0.06	1.32 ± 0.09^a	0.97 ± 0.06	1.35 ± 0.11^a
Dityrosine (U/mg protein)	0.36 ± 0.02	0.46 ± 0.04^a	0.34 ± 0.02	0.57 ± 0.05^a	0.40 ± 0.03	0.52 ± 0.04^a
Tryptophan (U/mg protein)	7.91 ± 0.45	7.43 ± 0.58	7.95 ± 0.51	7.19 ± 0.63^a	8.01 ± 0.55	7.42 ± 0.67
Sulfhydryl groups (nmol/mg protein)	4.11 ± 0.25	3.59 ± 0.31^a	4.07 ± 0.27	3.24 ± 0.30^a	4.09 ± 0.27	3.47 ± 0.30^a
Amino groups (nmol Tyr/mg protein)	47.2 ± 3.0	42.1 ± 3.1^a	47.6 ± 2.9	37.5 ± 3.1^a	46.1 ± 3.1	39.1 ± 3.3^a

^aSignificantly different from control group (p < 0.05).

Table 4 Activity of cathepsin B (pNA, μmol/g tissue) in the liver homogenate, cytosol, and lysosomes of control and F. hepatica-infected rats at 4, 7, and 10 wpi

	Weeks postinfection
	4		7		10
Localization	Control rats	Infected rats	Control rats	Infected rats	Control rats	Infected rats
Homogenate	1.87 ± 0.12	1.54 ± 0.13^a	1.84 ± 0.11	1.42 ± 0.12^a	1.89 ± 0.12	1.61 ± 0.14^a
Cytosol	0.22 ± 0.02	0.32 ± 0.03^a	0.22 ± 0.02	0.47 ± 0.04^a	0.22 ± 0.02	0.37 ± 0.03^a
Lysosomes	1.65 ± 0.09	0.99 ± 0.08^a	1.65 ± 0.09	0.95 ± 0.08^a	1.65 ± 0.09	1.24 ± 0.10^a

^a Significantly different from control group (p < 0.05).

Table 5 Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the serum of control and F. hepatica-infected rats at 4, 7, and 10 wpi

	Weeks postinfection
	4		7		10
	Control rats	Infected rats	Control rats	Infected rats	Control rats	Infected rats
ALT (U/L)	32.6 ± 1.4	93.7 ± 6.9^a	34.5 ± 1.4	96.1 ± 6.9^a	33.4 ± 1.6	98.3 ± 4.7^a
AST (U/L)	162.1 ± 6.5	357.1 ± 26.7^a	167.3 ± 6.5	361.8 ± 26.7^a	164.2 ± 6.9	359.3 ± 24.6^a

^aSignificantly different from control group (p < 0.05).