Changes in Phospholipid Composition Studied by HPLC and Electric Properties of Liver Cell Membrane of Ethanol-Poisoned Rats

Figures & data

Table 1 Effect of green and black tea on content of phospholipids and of their surface concentrations in liver cell membrane of rats intoxicated with ethanol

Groups	Phospholipids	Content of phospholipids of plasmalemma (mg/g tissues)	Surface concentration of phospholipids (10^−7 mol/m^2)	Groups	Content of phospholipids of plasmalemma (mg/g tissues)	Surface concentration of phospholipids (10^−7 mol/m^2)
Control 1	PI	0.023 ± 0.003	0.10 ± 0.01	Control 2	0.022 ± 0.002	0.09 ± 0.02
	PS	0.026 ± 0.003	0.12 ± 0.03		0.025 ± 0.004	0.12 ± 0.01
	PE	0.392 ± 0.023	1.74 ± 0.09		0.367 ± 0.020	1.63 ± 0.07
	PC	0.426 ± 0.031	2.00 ± 0.01		0.419 ± 0.010	1.96 ± 0.06
Green tea	PI	0.024 ± 0.008^b	0.10 ± 0.02^b	Black tea	0.022 ± 0.007^y	0.09 ± 0.04^y
	PS	0.027 ± 0.003^b	0.13 ± 0.01^b		0.026 ± 0.001^y	0.12 ± 0.05
	PE	0.401 ± 0.011^b	1.78 ± 0.07^b		0.397 ± 00.010^y	1.76 ± 0.09^y
	PC	0.432 ± 0.079^b	2.02 ± 0.09^b		0.427 ± 0.080^y	2.00 ± 0.08^y
Ethanol 1	PI	0.068 ± 0.010^a,^c	0.29 ± 0.02^a,^c	Ethanol 2	0.060 ± 0.009^x,^z	0.25 ± 0.06^x,^z
	PS	0.092 ± 0.019^a, c	0.43 ± 0.05^a,^c		0.090 ± 0.013^x,^z	0.42 ± 0.07^x,^z
	PE	0.920 ± 0.108^a,^c	4.08 ± 0.97^a,^c		0.898 ± 0.121^x,^z	3.98 ± 0.10^x,^z
	PC	1.299 ± 0.113^a,^c	6.09 ± 1.07^a,^c		1.311 ± 0.110^x,^z	6.15 ± 0.44^x,^z
Ethanol + green tea	PI	0.032 ± 0.012^b	0.14 ± 0.03^a,^b	Ethanol + black tea	0.047 ± 0.016^x,^z	0.20 ± 0.02^x,^z
	PS	0.050 ± 0.011^a^–^c	0.61 ± 0.06^a^–^c		0.078 ± 0.007^x,^z	0.36 ± 0.03^x,^z
	PE	0.490 ± 0.213^b	2.18 ± 0.09^a,^b		0.560 ± 0.073^x^–^z	2.49 ± 0.08^x^–^z
	PC	0.632 ± 0.113^a^–^c	2.96 ± 0.08^a^–^c		0.768 ± 0.101^x^–^z	3.61 ± 0.17^x^–^z

PI, phosphatidylinositol; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine.

Statistically significant differences for p < 0.05.

^aIn comparison with control 1.

^bIn comparison with ethanol group 1.

^cIn comparison with green tea group.

^xIn comparison with control 2.

^yIn comparison with ethanol group 2.

^zIn comparison with black tea group.

FIGURE 1 Effect of green (a) and black (b) tea on changes in electric properties of liver cell membrane in rats intoxicated with ethanol. (a): Statistically significant differences for p <0.05. ^a In comparison with control 1. ^b In comparison with ethanol group 1. ^c In comparison with the green tea group. (b): Statistically significant differences for p <0.05. ^x In comparison with control 2. ^y In comparison with ethanol group 2. ^z In comparison with the black tea group.

Table 2 Effect of green and black tea on changes of malondialdehyde measured as thiobarbituric reactive substances (TBARS) of liver cells in rats intoxicated with ethanol

	Control 1	Green tea	Ethanol	Ethanol + green tea	Control 2	Black tea	Ethanol	Ethanol + black tea
TBARS (nmol/g tissue)	47.8 ± 2.5	39.5 ± 2.7^a	81.5 ± 5.4^a,^c	63.1 ± 4.6^a^–^c	45.4 ± 2.7	39.9 ± 2.4^x	80.2 ± 6.2^x,^z	66.4 ± 5.1^x^–^z

Statistically significant differences for p < 0.05.

^aIn comparison with control 1.

^bIn comparison with ethanol group 1.

^cIn comparison with green tea group.

^xIn comparison with control 2.

^yIn comparison with ethanol group 2.

^zIn comparison with black tea group.