Chromatographic Examinations of Tea's Protection Against Lipid Oxidative Modifications

Figures & data

Table 1 The levels of catechins and theaflavins in the liver of 12-month-old rats receiving black tea, ethanol, and ethanol and black tea

	Groups
Parameter	Control	Black tea	Ethanol	Ethanol + black tea
EC (ng/g tissue)	n.d.	140 ± 9	n.d.	126 ± 10^b
EGC (ng/g tissue)	n.d.	231 ± 19	n.d.	213 ± 14^b
EGCG (ng/g tissue)	n.d.	246 ± 13	n.d.	218 ± 12^b
TF1 (ng/g tissue)	n.d.	22.4 ± 0.6	n.d.	21.0 ± 1.3^b
TF2A (ng/g tissue)	n.d.	57.2 ± 1.7	n.d.	54.0 ± 1.8^b
TF2B (ng/g tissue)	n.d.	17.2 ± 0.7	n.d.	15.6 ± 0.8^b
TF3 (ng/g tissue)	n.d.	20.4 ± 0.2	n.d.	18.8 ± 0.9^b

^aSignificantly different from control, p < 0.05.

^bSignificantly different from green tea group, p < 0.05.

^cSignificantly different from ethanol group, p < 0.05.

n.d., not detected.

Table 2 Antioxidative parameters in the liver of 12-month-old rats receiving black tea, ethanol, and ethanol and black tea

	Groups
Parameter	Control	Black tea	Ethanol	Ethanol + black tea
GSH-Px (U/mg protein)	31.8 ± 1.8	32.6 ± 1.7	24.0 ± 1.8^a,b	29.3 ± 1.7^a,b
GSH (μmol/g tissue)	0.99 ± 0.04	1.06 ± 0.06^a	0.73 ± 0.05^a,b	0.97 ± 0.06^a–c
Vitamin E (nmol/g tissue)	24.8 ± 1.6	25.9 ± 1.7	19.1 ± 1.4^a,b	24.4 ± 1.8^c

^a Significantly different from control, p <0.05.

^b Significantly different from green tea group, p <0.05.

^c Significantly different from ethanol group, p <0.05.

Table 3 The levels of lipid peroxidation products: conjugate dienes (CDs), lipid hydroxyperoxides (LOOHs), malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and 8-isoPGF_2α in the liver of 12-month-old rats receiving black tea, ethanol, and ethanol and black tea

	Groups
Parameter	Control	Black tea	Ethanol	Ethanol + black tea
CD (μmol/g tissue)	1.08 ± 0.02	0.89 ± 0.01^a	1.33 ± 0.13^a,b	1.26 ± 0.22^b
LOOH (μmol/g tissue)	13.60 ± 2.35	13.20 ± 2.77^a	42.60 ± 0.03^a,b	33.70 ± 0.09^a–c
MDA (nmol/g tissue)	1.12 ± 0.21	0.69 ± 0.20^a	1.68 ± 0.52^a,b	1.14 ± 0.23^b,c
HNE (nmol/g tissue)	2.25 ± 0.30	1.90 ± 0.25	3.39 ± 0.45^a,b	2.53 ± 0.33^b,c
8-isoPGF2α (ng/g tissue)	2.44 ± 0.31	2.28 ± 0.21	11.06 ± 0.62^a,b	9.52 ± 0.34^a–c

^aSignificantly different from control, p < 0.05.

^bSignificantly different from green tea group, p < 0.05.

^cSignificantly different from ethanol group, p < 0.05.

Table 4 The levels of lipid peroxidation products (CD, LOOH, MDA, and HNE) in the plasma, brain, lung, stomach, kidney, spleen, and intestine of 12-month-old rats receiving black tea, ethanol, and ethanol and black tea

		Paremeter
Tissue	Groups	CD (μmol/g tissue [mL])	LOOH (μmol/g tissue [mL])	MDA (nmol/g tissue [mL])	HNE (nmol/g tissue [mL])
Plasma	Control	0.20 ± 0.03	13.41 ± 1.70	0.81 ± 0.07	0.30 ± 0.02
	Black tea	0.15 ± 0.02^a	13.39 ± 2.03	0.74 ± 0.05	0.25 ± 0.03^a
	Ethanol	0.22 ± 0.02^b	21.01 ± 3.80^a,^b	1.08 ± 0.05^a,^b	0.43 ± 0.03^a,^b
	Ethanol + black tea	0.21 ± 0.02^b	15.30 ± 2.30^c	0.83 ± 0.14^c	0.36 ± 0.03^a–c
Brain	Control	0.96 ± 0.03	19.00 ± 3.16	0.52 ± 0.12	4.01 ± 0.27
	Black tea	0.90 ± 0.01^a,^b	15.98 ± 8.53^b	0.46 ± 0.11	2.58 ± 0.20^a
	Ethanol	0.99 ± 0.01^a	35.80 ± 3.67^a	0.63 ± 0.19^a,^b	7.17 ± 0.56^a
	Ethanol + black tea	0.97 ± 0.04^b	21.00 ± 7.4^b,^c	0.56 ± 0.12^b	4.19 ± 0.32^b,^c
Lung	Control	1.35 ± 0.03	23.63 ± 0.79	0.8 ± 0.07	0.40 ± 0.06
	Black tea	1.29 ± 0.08^a	23.50 ± 0.65	0.78 ± 0.09	0.38 ± 0.07
	Ethanol	1.49 ± 0.03^b	29.54 ± 0.83^b	0.95 ± 0.06^a,^b	0.51 ± 0.07^a,^b
	Ethanol + black tea	1.39 ± 0.05^b,^c	25.93 ± 0.58^a–c	0.84 ± 0.08	0.44 ± 0.04
Stomach	Control	0.90 ± 0.10	17.48 ± 0.58	0.46 ± 0.05	0.89 ± 0.10
	Black tea	0.87 ± 0.05	16.97 ± 0.67	0.40 ± 0.03	0.87 ± 0.07
	Ethanol	0.99 ± 0.15	24.73 ± 0.49^a,^b	0.58 ± 0.06^a,^b	1.13 ± 0.11^a,^b
	Ethanol + black tea	0.94 ± 0.05^b	18.09 ± 0.47^b,^c	0.49 ± 0.05^b,^c	0.94 ± 0.08^c
Kidney	Control	1.11 ± 0.06	25.90 ± 0.59	1.89 ± 0.10	0.73 ± 0.02
	Black tea	1.10 ± 0.08	23.86 ± 0.79^a	1.57 ± 0.10^a	0.72 ± 0.04
	Ethanol	1.14 ± 0.09	33.51 ± 1.03^a,^b	2.02 ± 0.20^b	0.84 ± 0.06^a,^b
	Ethanol + black tea	1.13 ± 0.03	27.74 ± 0.52^a–c	1.91 ± 0.08^b	0.78 ± 0.04^ab
Spleen	Control	1.16 ± 0.13	15.07 ± 0.10	0.90 ± 0.02	0.29 ± 0.05
	Black tea	1.04 ± 0.07	14.21 ± 0.57^a	0.81 ± 0.03^a	0.28 ± 0.04
	Ethanol	1.27 ± 0.18^b	29.17 ± 1.17^a,^b	1.24 ± 0.02^a,^b	0.36 ± 0.08
	Ethanol + black tea	1.17 ± 0.09^c	16.82 ± 0.57^a–c	1.12 ± 0.02^a–c	0.31 ± 0.07
Intestine	Control	0.88 ± 0.05	17.88 ± 3.17	0.62 ± 0.06	0.85 ± 0.04
	Black tea	0.80 ± 0.02	16.95 ± 0.64^a	0.57 ± 0.04	0.83 ± 0.09
	Ethanol	1.02 ± 0.22^a,^b	25.59 ± 1.30^b	0.69 ± 0.09^b	0.90 ± 0.08
	Ethanol + black tea	0.92 ± 0.14	18.52 ± 0.37^b,^c	0.65 ± 0.07^b	0.89 ± 0.07

^aSignificantly different from control, p <0.05.

^bSignificantly different from green tea group, p <0.05.

^cSignificantly different from ethanol group, p <0.05.