Decomposition of α-Tocopheryl Glycosides in Rat Tissues

Figures & data

FIGURE 1 Structure of α-tocopherol (1) and p-nitrophenyl glycosides (2b–5b).

FIGURE 2 Synthesis of α-tocopheryl O-glycosides.

FIGURE 3 Chromatograms of the extracts from liver tissue (from 50 μL of supernatant): a–d: endogenic α-tocopherol; e: after addition 10 μg of α-tocopherol. 5.7–6.0 free α-tocopherol peak.

Table 1 Amounts of p-nitrophenol (nmol) released after 60 min incubation of p-nitrophenyl glycosides 2b–5b in 50 μL of supernatant (n = 8)

Supernatant	2b (nmol) ± SD	3b (nmol) ± SD	4b (nmol) ± SD	5b (nmol) ± SD
Kidney	196 ± 40	10 ± 8	180 ± 20	278 ± 70
Intestine	38 ± 18	6 ± 3	42 ± 5	130 ± 40
Brain	18 ± 7	2.5 ± 0.5	20 ± 5	90 ± 30
Liver	70 ± 10	7.5 ± 0.6	60 ± 10	80 ± 15

Table 2 Amounts of released -tocopherol (nmol) after incubation of glycosides 2a–5a with 50 μL of supernatant fluid (n = 8)

	2a (nmol) ± SD		3a (nmol) ± SD		4a (nmol) ± SD		5a (nmol) ±S D
Supernatant	α-toc.^a	Δ3^b	α-toc.^a	Δ1^b	α-toc.^a	Δ2^b	α-toc.^a	Δ4^b	endo (nmol)^c
Liver	0.140 ± 0.5	–0.015 ± 0.0	0.921 ± 0.1	0.767 ± 0.23	3.217 ± 0.3	3.063 ± 0.45	0.156 ± 0.37	0.002 ± 0.00	0.154 ± 0.25
Kidney	0.923 ± 0.47	0.591 ± 0.17	1.072 ± 0.12	0.740 ± 0.2	1.034 ± 0.28	0.702 ± 0.4	0.327 ± 0.35	0.005 ± 0.00	0.332 ± 0.23
Intestine	2.276 ± 0.52	1.967 ± 0.2	2.618 ± 0.09	2.311 ± 0.25	2.502 ± 0.29	2.195 ± 0.42	0.323 ± 0.39	0.016 ± 0.00	0.307 ± 0.26
Brain	1.081 ± 0.49	0.696 ± 0.19	0.619 ± 0.11	0.234 ± 0.21	2.009 ± 0.31	1.624 ± 0.39	0.327 ± 0.37	–0.058 ± 0.00	0.385 ± 0.02

^aAmounts of α-tocopherol released after incubation of the appropriate glycoside 2a–5a.

^bAmounts of released α-tocopherol diminished by the endogenic content.

^cEndogenic content of α-tocopherol in 50 μL of supernatant fluid.

Table 3 Amounts of released α-tocopherol (nmol) after incubation of 2a–5a glycosides in citric buffer (n = 8)

	Glycoside (nmol)
Time of incubation (h)	2a ± SD	3a ± SD	4a ± SD	5a ± SD
2	0.100 ± 0.22^a	0.070 ±0.09^a	0.116 ± 0.04^a	0.078 ± 0.07^a
4	0.145 ± 0.43^a	0.098 ± 0.14^a	0.198 ± 0.04^a	0.124 ± 0.09^a
8	0.433 ± 0.325^a	0.350 ± 0.1^a	0.366 ± 0.09^a	0.471 ± 0.11^a
12	0.970 ± 0.09^a	0.584 ± 0.15^a	1.279 ± 0.08^a	0.528 ± 0.15^a
24	1.729 ± 0.47^a	0.926 ± 0.28^a	1.391 ± 0.08^a	0.602 ± 0.2^a

^aAmount of α-tocopherol released as a result of incubation of the glycosides 2a–5a in citric buffer.

FIGURE 4 The comparison of released α-tocopherol after 2-h incubation of the glycosides 2a–5a with 50 μL of the specified supernatants and the endogenic content of α-tocopherol. The results are presented as the mean value ± standard deviation (n = 20).

FIGURE 5 Amounts of α-tocopherol released from glycosides 2a–4a as a result of 2-h hydrolysis, diminished by the endogenic content α-tocopherol. The results (n = 10) are presented as the mean value ± standard deviation.

FIGURE 6 Amount of α-tocopherol released as a result of hydrolysis of the glycoside 2a–5a in buffer. The results (n = 10) were presented as the mean value ± standard deviation.