E-cigarette aerosols induce lower oxidative stress in vitro when compared to tobacco smoke

Figures & data

Table 1. AqE exposure concentrations used during the reported studies. Neat (100%) AqE equated to 10 puffs in 20 mL capture media or 0.5 puffs/mL.

AqE stock (%)	100	50	25	12.5	0.0
AqE puffs/mL	0.500	0.250	0.125	0.063	0.000

Table 2. QC data providing total nicotine (μg) and optical density (OD^320 nm) of the AqE and the CO generated during AqE generation (10 puffs in 20 mL capture media) (LOD = ±0.01% vol).

QC test/product	Nicotine (μg)	Carbon monoxide (mg)	Optical density (320 nm)
3R4F cigarette	124.26 ± 16.02	24.75 ± 2.29	0.749 ± 0.055
Cigilike device	92.69 ± 18.16	<LOD	0.463 ± −0.023
Closed modular device	100.6 ± 30.84	<LOD	0.459 ± 0.037
AqE capture medium	<LOD	N/A	0.446 ± 0.031

Table 3. Measurement of carbonyl-containing compounds in cigarette smoke and e-cigarette vapor.

Carbonyl concentration(μg/10 puffs)/product	Acetaldehyde	Acetone	Acrolein	Formaldehyde	Propionaldehyde	Crotonaldehyde	Methyl ethylketone	Butyraldehyde	Totalcarbonylcomponents
3R4F cigarette	1604	672	159	88	123	53	187	86	2972
Closed modular e-cigarette	10	6	6	12	<LOD	<LOD	<LOD	<LOD	35
% Reductions	99	99	96	86	100	100	100	100	99

Table 4. Measurement of carbonyl-containing compounds in AqE of cigarette smoke and e-cigarette vapor.

Carbonyl concentration (μg/10 puffs)/product	Acetaldehyde	Acetone	Acrolein	Formaldehyde	Propionaldehyde	Crotonaldehyde	Methyl ethyl ketone	Butyraldehyde	Totalcarbonylcomponents
3R4F cigarette	1045	395	72	42	77	48	136	<LOD	1815
Closed modular e-cigarette	<LOD	7	<LOD	10.8	<LOD	<LOD	<LOD	<LOD	18
% Reductions	100	98	100	74	100	100	100	N/A	99

Figure 1. NCI-H292 cell viability was reduced in a concentration-dependent manner in response to exposure to 3R4F AqE but not to the closed modular e-cigarette AqE (A) or cigilike AqE (B). The activation of apoptosis was increased by 3R4F AqE but not to the closed modular e-cigarette AqE (C) or cigilike AqE (D). Data are expressed as % change relative to vehicle control. Data are means of 12 replicates, repeated over a minimum of four independent experiments (±SD).

Figure 2. Intracellular glutathione ratios were lowered in NCI-H292 cells in response to exposure to 3R4F AqE but not to the closed modular e-cigarette AqE (A) or cigilike AqE (B). Intracellular oxidant species generation was increased in H292 cells in response to exposure to 3R4F AqE but not to closed modular AqE (C) or cigilike AqE. Data are expressed as % change relative to vehicle control. Data are means of 12 replicates, repeated over a minimum of four independent experiments (±SD).

Figure 3. The transcriptional activation of the ARE in the GloResponse™ ARE-luc2P H292 cell-line was increased in response to exposure to combustible cigarette AqE but not to closed modular AqE (A) or cigilike AqE (B). Data are expressed as % change relative to vehicle control. Data are means of 12 replicates, repeated over a minimum of four independent experiments (±SD).