Alveolar Macrophage Recruitment and Activation by Chronic Second Hand Smoke Exposure in Mice

Figures & data

Table 1 Environmental conditions at each time point of exposure

Month	Exposure	Relative humidity	Temp	CO ppm	nicotine mg/m^3	TSP mg/m^3
1	Chamber 2 Medium dose SHS	56 ± 12%	71.2 ± 0.7	238 ± 62	2.5 ± 0.4	69 ± 21d
1	Chamber 1 High dose SHS	56 ± 12%	71.2 ± 0.7	303 ± 72	4.8 ± 1.6	98 ± 27
2	Chamber 1 High dose SHS	59 ± 11%	71.2 ± 0.7	351 ± 83	5.5 ± 2.6	117 ± 32
3	Chamber 1 High dose SHS	60 ± 11%	71.1 ± 0.7	394 ± 95	6.8 ± 2.7	131 ± 34

Data are mean ± standard deviation.

Figure 1 No differences in weight gain were measured in mice exposed to high dose SHS. Filtered air- and high-dose SHS-exposed mice were weighed at 2-week intervals throughout the exposure. Male mice weighed more than female mice at every time point. There were no statistically significant differences in the amount of weight gain between filtered air- and SHS-exposed mice. Data are expressed as mean weight in grams ± SD; n = 13–15 for each group at weeks 0–4, n = 9–12 for each group at weeks 6–8 and n = 7–9 for each group at week 10.

Figure 2 BAL total inflammatory cell and macrophage numbers were increased in high dose SHS-exposed mice compared to filtered air-exposed. Cell counts and differentials were determined in BAL fluid from filtered air- and medium- or high- dose SHS-exposed mice after 1, 2 or 3 months of exposure. p-values are for comparison with filtered air-exposed control at that time point. Data are expressed as median (line), interquartile range (box) and range (whiskers).

Table 2 Induction of selected markers of alveolar macrophage activation with SHS exposure.

	1 Month				2 Month		3 Month
	Medium SHS		High SHS		High SHS		High SHS
Gene	Fold-induction	p-value	Fold-induction	p-value	Fold-induction	p-value	Fold-induction	p-value
Mmp12	1.4	0.78	0.9	0.16	0.35	0.26	0.7	0.044
Pla2g7	3.9	0.0006	3.1	0.0001	3.16	0.0004	4.6	0.0001
Trem2	1.2	0.13	1.3	0.09	1.59	0.0006	1.4	0.0006
Pirin	1.4	0.056	1.3	0.02	1.56	0.007	1.5	0.0001
Dkfzp434l142	1.0	0.36	1.4	0.85	1.81	0.002	1.4	0.007

Figure 3 Protein levels of CCL2 and TNFα were increased in BAL fluid from mice exposed to high dose SHS. CCL2 and TNFα were measured using ELISA in BAL fluid from filtered air- and medium- or high- dose SHS-exposed mice at after 1, 2 or 3 months of exposure. N = 5–6 for each group except for month 3, at which n = 16 for each group. Data are expressed as median (line), interquartile range (box) and range (whiskers, with extreme values as open circles); *denotes p < 0.05 as compared to air-exposed control. Med SHS = medium dose SHS.