Effect of Methanol Intoxication on Rat Neutrophil Functions

Figures & data

FIG. 1 Body weights of rats that did not receive SRBC treatment. Values are expressed as Mean ± SD from 6 animals. *p < 0.05 significance vs. control groups.

FIG. 2 Body weights of rats that received SRBC injection. Values are expressed as Mean ± SD from 6 animals. *p < 0.05 significance vs. control groups.

TABLE 1 Lymphoid organ weights in rats from the various treatment groups

	SRBC-untreated			SRBC-treated
		15-day	30-day		15-day	30-day
Organs (mg)	Control	MeOH	MeOH	Control	MeOH	MeOH
Spleen	1,223 ± 54	910 ± 63	696 ± 83^@	1,381 ± 27	1,032 ± 39	839 ± 35^@
Thymus	232 ± 12	171 ± 7	121 ± 10^@	260 ± 9	172 ± 10	130 ± 24^@
Lymph node	32 ± 2	24 ± 3	16 ± 2^@	39 ± 2	28 ± 1	23 ± 1^@

Values are expressed as mean ± SD from 6 animals

*Value significantly different from the respective control (p < .05)

^@Value significantly different between the 15- and 30-day treatment groups (p < 0.05)

TABLE 2 MeOH effects on total and differential leukocyte counts of SRBC-untreated rats

Parameters	Control	15-day MeOH	30-day MeOH
TLC Total counts/mm^3.	23,367 ± 946	16,592 ± 1219	13,283 ± 25,53^@
DLC Neutrophils (%)	24 ± 8	21 ± 3	16 ± 3
Lymphocytes (%)	71 ± 7	76 ± 3	79 ± 5

Values are expressed as mean ± SD from 6 animals

*Value significantly different from the respective control (p < 0.05)

^@Value significantly different between the 15- and 30-day treatment groups (p < 0.05)

TABLE 3 Effect of MeOH on ex vivo neutrophil functions of SRBC-untreated rats

Parameter assay	Control	15-day MeOH	30-day MeOH
Phagocytic index (PI) (%)	91.0 ± 2.0	80.0 ± 4.0	79.0 ± 2.0
Avidity index (AI)	2.6 ± 0.3	3.2 ± 0.5	3.2 ± 0.1
NBT reduction (%)	6.3 ± 2.0	18.2 ± 2.0	15.0 ± 1.0^@
Adherence (%)	49.0 ± 5.0	44.0 ± 5.0	29.5 ± 5.0^@

Values are expressed as mean ± SD from 6 animals

*Value significantly different from the respective control (p < 0.05)

^@Value significantly different between the 15- and 30-day treatment groups (p < 0.05)

TABLE 4 MeOH effects on total and differential counts of SRBC-treated rats

Parameters	Control	15-day MeOH	30-day MeOH
TLC Total count/mm^3	18,633 ± 2057	16,675 ± 1908	14,067 ± 930^@
DLC Neutrophils (%)	8 ± 3	23 ± 4	15 ± 5^@
Lymphocytes (%)	89 ± 4	78.5 ± 4	82 ± 6

Values are expressed as mean ± SD from 6 animals

*Value significantly different from the respective control (p < 0.05)

^@Value significantly different between the 15- and 30-day treatment groups (p < 0.05)

TABLE 5 Effect of MeOH on ex vivo neutrophil functions in SRBC-treated rats

Parameters	Control	15-day MeOH	30-day MeOH
Phagocytic index (PI) (%)	87.0 ± 4.0	68.0 ± 3.0	63.0 ± 4.0
Avidity index (AI)	4.1 ± 0.1	2.6 ± 0.3	2.1 ± 0.3
NBT reduction (%)	32.0 ± 3.3	22.0 ± 3.0	19.0 ± 2.4
Adherence (%)	78.0 ± 9.2	52.0 ± 9.0	30.0 ± 4.3^@

Values are expressed as mean ± SD from six animals

*Value significantly different from the respective control (p < 0.05)

^@Value significantly different between the 15- and 30-day treatment groups (p < 0.05)