All-E lutein and 3′-epilutein in the epidermis of chronic arsenic poisoning

Figures & data

Fig. 1.  HPLC profile of the carotenoids in skin biopsies of arsenic non-exposed and raindrop-shaped discolored spots of chronic arsenic poisoning. Peak 1: all-E lutein (retention time- 10.9 min); peak 2: 13-Z zeaxanthin (13.3 min); peak 3: all-E zeaxanthin (13.9 min); peak 4: 3′-epilutein (15.5 min); peak 5: 9-Z lutein (18.0 min); peak 6: 9′-Z lutein (18.7 min); peak 7: 2′,3′-anhydrolutein (19.5 min); and peak 8: cryptoxanthin (22.4 min).

Table 1. Concentration of all-E lutein and 3′-epilutein in the epidermis of normal volunteers and patients of chronic arsenic poisoning

			Patients of chronic arsenic poisoning
	Normal volunteers		Dark brown spot		Discolored spot
Number	All-E lutein (microgram/g)	3′-epilutein (microgram/g)	All-E lutein (microgram/g)	3′-epilutein (microgram/g)	All-E lutein (microgram/g)	3′-epilutein (microgram/g)
1	1.19	0.80
2	0.86	1.21
3	1.13	0.76
4	1.30	0.46
5	0.82	0.32
6	1.62	0.90
7	1.15	0.88
8	0.99	0.62
9	0.78	0.52
10			0.25	0.11	1.12	0.31
11			0.46	0.40	1.26	0.82
12			0.20	0.12	0.51	0.81
13			0.25	0.16	1.17	0.47
14			0.22	0.12	1.10	0.19
15			0.40	0.21	0.81	0.31
16			0.24	0.19	0.67	0.19
17			0.21	0.14	0.47	0.20
18			0.44	0.42	0.70	0.57
Mean	1.09	0.71	0.29	0.20	0.86	0.43
S.D.	0.26	0.27	0.10	0.11	0.29	0.25