Systemic envenomation caused by the wandering spider Phoneutria nigriventer, with quantification of circulating venom

Figures & data

Fig. 1.  Female P. nigriventer responsible for the bite.

Fig. 2.  Standard curve for the ELISA used to quantify P. nigriventer venom. The points represent the means of duplicate determinations that differed from each other by < 5% in each assay. Similar results were obtained in four additional assays.

Table 1.  Accuracy and intra- and inter-assay variation for the ELISA used to quantify P. nigriventer venom

Venom concentration (ng/mL)		Accuracy (%) (n = 6)	Intra-assay CV (%) (n = 6)	Inter-assay CV (%) (n = 3)
Theoretical	Measured
20	19.5 ± 0.9	97.5	4.1	4.7
40	41.1 ± 0.9	102.7	5.8	2.2
80	75.0 ± 1.6	93.7	2.9	2.1

Venom concentrations expressed as means ± SD (n = 6). CV, coefficient of variation.