https://orcid.org/0000-0001-8467-2557
Figures & data
Table 1. Product and formulation characteristics of the 5-grass pollen and 1-grass pollen SLIT tablets
Figure 1. Kinetics of allergen activity release and dissolution of 300 IR and 100 IR HDM and 5 grass SLIT tablets in vitro.
An in vitro dissolution assay endorsed by the European Pharmacopoeia (Ph. Eur. 2.9.3 dissolution test for solid dosage forms, apparatus 4 flow-through cell [Citation31]) was used to investigate the dissolution time of 6 replicate samples of the 300 IR or 100 IR HDM and 5 grass pollen SLIT tablets, and the amount of allergenic activity released at each time point was measured using an ELISA-based assayELISA, enzyme-linked immunosorbent assay; HDM, house dust mite; IR, index of reactivity; SLIT, sublingual immunotherapy.
Figure 2. Kinetics of grass pollen allergen binding by oromucosal Langerhans cells ex vivo.
This was an ex vivo study not related to SLIT tablet use. Using oromucosal tissue from individuals with grass pollen allergy (n = 5) or non-atopic individuals (n = 5), incubated with fluorescein isothiocyanate-coupled Phl p 5 (1 mg/mL) at 37°C for the specified times, significant binding of Phl p 5 to Langerhans cells was detected from 5°minutes of allergen exposure onwards, and binding capacity was comparable between tissue samples. Figure adapted from Allam et al. 2010 [Citation32].SLIT, sublingual immunotherapy.
