Cellular, matrix, and growth factor components of the joint capsule are modified early in the process of posttraumatic contracture formation in a rabbit model

Figures & data

Primary antibodies used in western blot

Monoclonal antibody to:	Host	Company
α-SMA	Mouse	Sigma
GAPDH	Mouse	Chemicon
MMP-1	Mouse	Lab Vision
MMP-13	Mouse	Lab Vision
TIMP-1	Mouse	Lab Vision
collagen I	Mouse	Lab Vision
collagen III	Mouse	Sigma
TGF-β1	Mouse	Sigma

α-SMA: α-smooth muscle actin;

GAPDH: glyceraldehyde-3-phosphate dehydrogenase;

MMP: matrix metalloproteinase;

TIMP: tissue inhibitors of matrix metalloproteinase;

TGF-β₁: transforming growth factor-β₁.

Figure 1. Levels of α-SMA mRNA and protein in joint capsules. (α-SMA is a myofibroblast marker). The values are normalized to GAPDH mRNA and protein.

Figure 2. Levels of collagen mRNA and protein in joint capsules. The values are normalized to GAPDH mRNA and protein.

Figure 3. Levels of MMP mRNA and protein in joint capsules. The values are normalized to GAPDH mRNA and protein.

Figure 4. Levels of TIMP mRNA and protein in joint capsules. The values are normalized to GAPDH mRNA and protein.

Figure 5. Levels of growth factor mRNA and protein in joint capsules. The values are normalized to GAPDH mRNA and protein.