Impact of selenium supplementation on productive performance and egg selenium status in native Aseel chicken

Figures & data

Table 1. Ingredients and chemical composition of basal feeds.

	Ration-I (Pre-breeder)	Ration-II (Layer breeder)	Ration-III (Male breeder)
Ingredients, %
Maize	42.61	63.13	39.40
Rice tips	19.00	–	31.00
Wheat bran	13.00	–	15.80
Soybean meal 48%	15.62	27.11	10.45
Corn gluten 65%	1.00	–	–
Dicalcium phosphate	1.20	1.50	0.70
Sodium chloride	–	0.30	–
Limestone	7.42	7.60	2.65
Vitamin mineral premix^a	–	0.30	–
DL-Methionine	0.15	0.06	–
Total	100	100	100
Analysed chemical composition
Crude Protein, %	15.22	17.54	13.29
ME by calculation^b, MJ/kg	11.22	11.43	11.94
Calcium, %	2.71	3.19	1.12
Phosphorus, %	0.36	0.42	0.22
Lysine, %	0.84	0.87	0.74
Methionine, %	0.44	0.46	0.39
Analysed selenium, ppm	0.06	0.10	0.04

^a Premix per kg compound feed: Vitamin A 12,000 U; Vitamin D₃ 2200 ICU; Vitamin E 10 mg; Vitamin K 32 mg; Vitamin B₁ 1 mg; Vitamin B₂ 4 mg; Vitamin B₆ 1.5 mg; Vitamin B₁₂ 10 μg; Nicotinic acid 20 mg; Folic acid 1 mg; Pantothenic acid 10 mg; Biotin 50 μg; Choline chloride 500 mg; Copper 10 mg; Iron 30 mg; Manganese 55 mg; Zinc 50 mg.

^b Metabolizable energy was estimated according to NRC (1994).

Table 2. Composition of supplemental and analysed selenium^a (ppm).

Experimental Rations	Ration-I (pre-breeder)			Ration-II (layer breeder)			Ration-III (male breeder)
Treatment units	A	B	C	A	B	C	A	B	C
Supplemental level of Se-Y	0.30	0.00	0.00	0.30	0.00	0.00	0.30	0.00	0.00
Supplemental level of Sod-Sel	0.00	0.30	0.00	0.00	0.30	0.00	0.00	0.30	0.00
Analysed Se in basal diets	0.00	0.00	0.06	0.00	0.00	0.10	0.00	0.00	0.04
Analysed level of Se-Y	0.33	0.00	0.00	0.34	0.00	0.00	0.32	0.00	0.00
Analysed level of Sod-Sel	0.00	0.32	0.00	0.00	0.33	0.00	0.00	0.31	0.00

^a Analyzed selenium level in diets = Analysed selenium in basal diet + Analysed selenium in supplemented diet; Se: selenium; Se-Y: selenium yeast (organic Se source); Sod-Sel: sodium selenite (inorganic Se source); A: treatment group, fed with Se-Y supplemented ration; B: treatment group, fed with Sod-Sel supplemented ration; C: control group, fed with basal ration without Se supplement.

Table 3. Impact of different selenium sources and Aseel varieties on growth and production parameters in the pullets of indigenous Aseel.

Variables	DFI g ± SEM	BW g ± SEM	AAM d ± SEM	EP % ± SEM	EM g ± SEM	FCR/dozen eggs ± SEM	FCR/kg egg mass ± SEM
Se sources (n = 28)
Se-Y	88.30 ± 0.11^a	1973.56 ± 3.43^a	168.61 ± 0.64^c	38.17 ± 1.27^a	112.52 ± 2.63^a	3.26 ± 0.06^c	6.77 ± 0.23^c
Sod-Sel	85.96 ± 0.07^b	1954.61 ± 3.22^b	184.03 ± 1.49^b	28.07 ± 0.59^b	82.06 ± 1.59^b	4.21 ± 0.07^b	8.33 ± 0.18^b
C	85.78 ± 0.08^b	1960.06 ± 3.47^b	205.67 ± 1.71^a	22.44 ± 0.63^c	63.84 ± 1.28^c	4.81 ± 0.06^a	10.12 ± 0.24^a
Varieties of indigenous Aseel (n = 21)
LA	86.80 ± 0.25^ab	1958.70 ± 4.74^b	186.33 ± 3.42^a	28.80 ± 1.56^bc	86.77 ± 3.23^ab	4.08 ± 0.16^b	8.16 ± 0.46^b
MK	86.55 ± 0.26^b	1955.04 ± 3.91^b	186.85 ± 3.74^a	27.31 ± 1.67^c	78.29 ± 3.60^d	4.29 ± 0.19^a	9.01 ± 0.43^a
PS	86.54 ± 0.28^b	1967.60 ± 4.05^a	185.38 ± 3.78^a	30.51 ± 2.34^ab	88.25 ± 6.34^a	3.93 ± 0.23^b	8.01 ± 0.55^b
MN	86.82 ± 0.29^a	1969.64 ± 3.55^a	185.85 ± 4.09^a	31.62 ± 2.82^a	81.80 ± 4.82^bc	4.07 ± 0.20^b	8.16 ± 0.41^b

Different alphabets on means show significant (p ≤.05) difference; SEM: standard error of means; DFI: daily feed intake; BW: body weight; AAM: age at maturity; EP: egg production; EM: egg mass; Se-Y: Se-enriched yeast; Sod-Sel: sodium selenite; C: control (without Se supplement); LA: Lakha; MK: Mushki; PS: Peshaweri; MN: Mianwali; n: number of observations.

Table 4. Impact of interaction of Aseel varieties and selenium sources on production performance in the pullets of indigenous Aseel.

Variables	DFI g ± SEM	BW g ± SEM	AAM d ± SEM	EP % ± SEM	EM g ± SEM	FCR/dozen eggs ± SEM	FCR/kg egg mass ± SEM
Varieties of indigenous Aseel × Se sources (n = 7)
LA
Se-Y	88.25 ± 0.33^a	1948.64 ± 9.33^bc	170.57 ± 1.29^c	34.15 ± 1.68^b	93.35 ± 1.28^b	3.42 ± 0.10^d	6.76 ± 0.22^de
Sod-Sel	86.29 ± 0.15^b	1955.19 ± 6.17^abc	185.57 ± 2.87^b	28.80 ± 1.68^c	86.64 ± 2.67^c	4.14 ± 0.10^c	8.27 ± 0.49^c
C	85.97 ± 0.16^bcd	1972.27 ± 7.08^a	202.86 ± 3.85^a	23.45 ± 1.47^de	68.78 ± 1.66^e	4.70 ± 0.13^ab	9.89 ± 0.78^a
MK
Se-Y	88.12 ± 0.16^a	1949.71 ± 5.54^bc	170.71 ± 1.08^c	33.93 ± 1.58^b	94.75 ± 3.01^b	3.48 ± 0.09^d	7.71 ± 0.39^cd
Sod-Sel	85.89 ± 0.13^bcd	1946.60 ± 4.73^c	182.28 ± 2.98^b	26.56 ± 0.98^cd	80.43 ± 2.26^c	4.40 ± 0.12^bc	8.54 ± 0.46^bc
C	85.58 ± 0.13^cd	1968.80 ± 7.21^ab	207.57 ± 3.32^a	21.43 ± 1.09^e	59.69 ± 1.28^a	5.02 ± 0.05^a	10.77 ± 0.16^a
PS
Se-Y	88.29 ± 0.10^a	1956.40 ± 5.35^abc	167.14 ± 1.06^c	40.85 ± 0.98^a	125.70 ± 2.99^a	2.96 ± 0.08^e	5.85 ± 0.15^e
Sod-Sel	85.86 ± 0.13^bcd	1970.17 ± 6.87^ab	184.14 ± 3.28^b	27.45 ± 1.22^c	83.80 ± 3.99^c	4.14 ± 0.21^c	8.16 ± 0.29^c
C	85.48 ± 0.05^d	1976.23 ± 7.35^a	204.85 ± 3.45^a	23.22 ± 1.31^de	64.86 ± 3.51^e	4.70 ± 0.13^ab	10.03 ± 0.58^a
MN
Se-Y	88.56 ± 0.20^a	1976.95 ± 6.92^a	166.00 ± 0.75^c	43.75 ± 1.41^a	126.50 ± 4.16^a	3.20 ± 0.08^de	6.76 ± 0.46^de
Sod-Sel	85.81 ± 0.13^bcd	1963.68 ± 4.05^abc	184.14 ± 3.28^b	29.46 ± 0.36^c	77.35 ± 2.51^d	4.19 ± 0.07^c	8.33 ± 0.37^c
C	86.09 ± 0.16^bc	1968.29 ± 6.83^ab	207.42 ± 3.54^a	21.65 ± 1.31^e	61.79 ± 1.68^ef	4.83 ± 0.09^a	9.73 ± 0.36^ab

Different alphabets on means show significant (p ≤ .05) difference; SE: standard error of means; DFI: daily feed intake; BW: body weight; AAM: age at maturity; EP: egg production; EM: egg mass; Se-Y: Se-enriched yeast; Sod-Sel: sodium selenite; C: control (without Se supplement); LA: Lakha; MK: Mushki; PS: Peshaweri; MN: Mianwali; n: number of observations.

Table 5. Impact of selenium sources and Aseel varieties on egg selenium concentration in the pullets of indigenous Aseel.

Variables	Se content in egg yolk μg ± SEM	Se content in egg albumen μg ± SEM	Se content in whole egg μg ± SEM
Se sources (n = 12)
Se-Y	8.31 ± 0.01^a	3.33 ± 0.01^a	11.70 ± 0.01^a
Sod-Sel	6.50 ± 0.01^b	2.60 ± 0.01^b	9.13 ± 0.01^b
C	5.52 ± 0.01^c	2.22 ± 0.01^c	7.77 ± 0.01^c
Varieties of indigenous Aseel (n = 9)
LA	6.78 ± 0.40^ab	2.72 ± 0.16^a	9.53 ± 0.57^a
MK	6.76 ± 0.40^b	2.71 ± 0.16^a	9.51 ± 0.57^a
PS	6.80 ± 0.41^a	2.72 ± 0.16^a	9.55 ± 0.57^a
MN	6.78 ± 0.41^ab	2.71 ± 0.16^a	9.53 ± 0.57^a

Different alphabets on means show significant (p ≤ .05) difference; SE: standard error of means; Se: selenium; Se-Y: Se-enriched yeast; Sod-Sel: sodium selenite; C: control (without Se supplement); LA: Lakha; MK: Mushki; PS: Peshaweri; MN: Mianwali; n: number of observations.

Table 6. Impact of interaction of Aseel varieties and selenium sources on egg selenium concentration in the pullets of indigenous Aseel.

Variables	Se content in egg yolk μg ± SEM	Se content in egg albumen μg ± SEM	Se content in whole egg μg ± SEM
Varieties of indigenous Aseel × Se sources (n = 3)
LA
Se-Y	8.31 ± 0.01^a	3.33 ± 0.01^a	11.70 ± 0.01^a
Sod-Sel	6.50 ± 0.02^b	2.61 ± 0.01^b	9.14 ± 0.02^b
C	5.52 ± 0.02^c	2.21 ± 0.01^c	7.77 ± 0.03^c
MK
Se-Y	8.29 ± 0.01^a	3.32 ± 0.01^a	11.67 ± 0.01^a
Sod-Sel	6.47 ± 0.02^b	2.59 ± 0.01^b	9.10 ± 0.03^b
C	5.52 ± 0.03^c	2.21 ± 0.01^c	7.76 ± 0.03^c
PS
Se-Y	8.34 ± 0.01^a	3.34 ± 0.01^a	11.73 ± 0.03^a
Sod-Sel	6.51 ± 0.01^b	2.60 ± 0.01^b	9.14 ± 0.01^b
C	5.53 ± 0.01^c	2.22 ± 0.01^c	7.79 ± 0.02^c
MN
Se-Y	8.33 ± 0.01^a	3.32 ± 0.01^a	11.70 ± 0.02^a
Sod-Sel	6.49 ± 0.01^b	2.60 ± 0.01^b	9.13 ± 0.02^b
C	5.51 ± 0.01^c	2.21 ± 0.01^c	7.75 ± 0.01^c

Different alphabets on means show significant (p ≤ .05) difference; SE: standard error of means; Se: selenium; Se-Y: Se-enriched yeast; Sod-Sel: sodium selenite; C: control (without Se supplement); LA: Lakha; MK: Mushki; PS: Peshaweri; MN: Mianwali; n: number of observations.

NRC. 1994. Nutrient requirements of poultry. 9th ed. Washington (DC): National Academy Press.

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