A modified vitrification method reduces spindle and chromosome abnormalities

Figures & data

Table 1. Patients’ characteristics and oocytes survival rates by groups.

	37°C group	RT group	P value
Age (years)	32.3 ± 3.2	31.7 ± 3.9	0.54
BMI (kg/m2)	23.2 ± 3.3	22.8 ± 3.1	0.56
Basic FSH (mIU/ml)	7.32.5	7.2 ± 2.6	0.86
Survival rate (%)	88.0% (44/50)	82.4% (42/51)	0.58
Pre-equilibrium time (min)	2.8 ± 0.90	5.2 ± 1.2	0.00

RT: room temperature.

Figure 1. Spindle (green) configuration and chromosomal (blue) alignment in human MII oocytes. (A1-3) Representative normal spindle and chromosomal configuration in human MII oocytes in the 37°C group; (B1-3 and C1-3) representative abnormal spindle and chromosomal configuration in the RT group. Arrows indicate the spindle multipoles and displaced chromosomes. Scale bar = 10 µm. MII: metaphase II; RT: room temperature.

Figure 2. Abnormal spindle, abnormal chromosome, and diffuse mitochondrial distribution rate in the two groups. (A) Representative abnormal spindle rate; (B) representative abnormal chromosome rate; (C) representative diffuse mitochondria rate. The proportion of spindle and abnormal chromosome in the 37°C group was significantly lower than that in the RT group. * indicates p < 0.05; RT: room temperature.

Figure 3. Mitochondrial (red) distribution in human MII oocytes. (A1-3) Peripheral mitochondrial distribution in the MII phase human oocytes in the 37°C group; (B1-3 and C1-3) semiperipheral and diffuse mitochondrial distribution in the RT group, respectively. Scale bar = 10 µm. MII: metaphase II; GV: germinal vesicle; RT: room temperature.