Figures & data
Figure 1. Effect of concentration of baby rabbit complement (BRC) on non-specific killing (NSK) of group B streptococcus (GBS) serotype V. (A) Opsonic indexes (OIs) and NSK ratios at different BRC concentrations in the GBS multiplexed opsonophagocytic killing assay (MOPA). (B) GBS-MOPA results obtained using different BRC concentrations ranging from 7.0% to 12.5%.
Figure 2. Specificity analysis of the group B streptococcus (GBS) multiplexed opsonophagocytic killing assay (MOPA). Specificity of the GBS-MOPA was analyzed by comparing pre-adsorption (open circle) and post-adsorption (closed circle) opsonic indexes (OIs) of the serum samples with homologous/heterologous GBS. Results with homologous adsorption are presented in the graphs. The y-axis and x-axis indicate the number of bacteria and the three-fold serum dilution number, respectively.
Figure 3. Comparison of opsonic indexes (OIs) obtained from the group B streptococcus (GBS) multiplexed opsonophagocytic killing assay (MOPA) versus those from the GBS single opsonophagocytic killing assay (SOPA). Twenty serum samples from subjects who had recovered from previous GBS infection (red circles) and 15 serum samples from healthy adults without any prior episode of GBS infection (blue circles) were tested in the MOPA (y-axis) and SOPA (x-axis). The dashed line and solid line indicate the line of identity and two-fold deviation from identity, respectively. OIs were estimated to be below the detection limit (assigned as 2, purple circle) in 13 samples (3 from subjects with previous GBS infection and 10 from healthy subjects) for serotype Ia and in 3 samples (3 from healthy subjects) for serotype V.
Table 1. Inter-assay precision of the group B streptococcus multiplexed opsonophagocytic killing assay (GBS-MOPA).
Table 2. Bacterial strains used in the group B streptococcus multiplexed opsonophagocytic killing assay (GBS-MOPA).