Figures & data
Figure 1. Dynamic viscosity of buffer and buffer containing 4 vol% capsules. The viscosity of buffer containing 4 vol% capsules (spheres) as well as the viscosity of pure buffer (squares) was measured and compared to each other. Error bars represent standard deviations with n = 6.
Figure 2. Oxygen transport capacities of pure KH-buffer (dark grey) and KH-buffer containing 4 vol% capsules (light grey) were measured at pO2 = 713 mmHg and compared to each other with *P < 0.05. Error bars represent standard deviations with n = 25.
Figure 3. Gas exchange in a nanocapsule dispersion. Three cycles of oxygen-loading and release are shown over a time period of 300 min (x-axis). The data were observed under purging with O2 (dark grey squares) and with N2 (black dots) of a buffer solution containing 4 vol% capsules. The 19F chemical shift (right y-axis) was measured and related to the degree of O2 saturation (left y-axis) via a calibration curve.
Figure 4. Determination of the ideal dose. After the initial stabilization period (flow 100%), the flow was reduced (flow 50%). For dose determination, control-heart (dark grey) was perfused with pure buffer compared to capsule-heart (light grey), which was perfused with buffer containing 2 vol% (first treatment), 4 vol% (second treatment), and 6 vol% (third treatment) capsules. Wash-out steps were performed with pure buffer. The initial LVDP was set to 100%. n = 1.
Figure 5. Effect of a repeated treatment on LVDP and RPP. After the initial stabilization period (flow 100%), the flow was reduced (flow 50%). Control-hearts (dark grey) were perfused with pure buffer and capsule-hearts (light grey) with buffer containing 4 vol% capsules. Wash-out periods were performed with pure buffer. LVDP and RPP were measured. Error bars represent standard deviations with n = 6.With P < 0.05 no significant differences were detected if compared to correspondent control-heart.
Figure 6. Consequence of a massive flow reduction on LVDP and RPP. After the initial stabilization period (flow 100%), the flow was reduced stepwise by 20% to 80%, 60%, 40% and 20%, respectively, of initial flow. At the end of the experiment, the initial flow of 100% was re-established. Control-hearts (dark grey) were perfused with pure buffer and capsule-hearts (light grey) with buffer containing 4 vol% capsules. LVDP and RPP were measured. Error bars represent standard deviations with n = 6, *P < 0.05 as compared to correspondent control-heart.
