Furanocoumarins from Kaffir lime and their inhibitory effects on inflammatory mediator production

Figures & data

Figure 2. The in vitro NO-scavenging activity of the fractions, sub-fractions and compounds 1 and 2.

The data were expressed as mean ± SD; n = 3. The different letters following the data show the significant content differences at p ≤ 0.05.

Figure 1. The structures of compounds 1 and 2.

Table 1. The ¹H and ¹³C NMR spectral data of 1 and 2 in CDCl₃ (δ in ppm, J in Hz)

Position	6ˊ,7ˊ-dihydroxybergamottin (1)		Oxypeucedanin hydrate (2)
	δ H	δC	δ H	δC
2		161.4		161.1
3	6.27 (d, 10.0)	112.5	6.31 (d, 9.6)	113.2
4	8.15 (d, 10.0)	139.6	8.18 (d, 10.0)	138.9
5		148.8		148.4
6		114.2		114.3
7		158.1		158.1
8	7.15 (s)	94.3	7.18 (s)	94.9
9		152.6		152.5
10		107.5		107.3
11	6.95 (d, 2.0)	105.1	6.99 (d, 1.6)	104.7
12	7.60 (d, 2.0)	144.0	7.61 (d, 2.4)	145.3
1’	4.95 (d, 6.8)	69.6		74.5
1ʹa			4.45 (dd, 9.6, 3.2)
1ʹb			4.54 (dd, 9.6, 8.0)
2’	5.60 (t, 6.8)	119.3	3.91 (dd, 7.6, 3.2)	76.5
3’		142.9		71.7
4’	2.35 (m)	36.5	1.36 (s)	26.7
5’	1.55 (m)	29.4	1.32 (s)	25.1
6’	3.32 (d, 10.0)	77.9
7’		73.1
8’	1.20 (s)	23.3
9’	1.17 (s)	26.6
10’	1.71 (s)	16.7

Table 2. The 50% inhibition concentrations of compounds 1 and 2 on NO, iNOS and COX-2 production

Sample	IC50 (μg/mL)
	NO	iNOS	COX-2
			HCT 116	HT-29
Compound 1	16.16 ± 1.08 ^b	18.63 ± 1.42 ^b	17.53 ± 0.88 ^a	18.19 ± 0.95 ^b
Compound 2	18.23 ± 1.25 ^b	22.54 ± 1.56 ^c	23.24 ± 1.05 ^b	22.27 ± 1.14 ^c
Curcumin	12.52 ± 0.63 ^a	15.55 ± 1.34 ^a	16.14 ± 0.65 ^a	14.29 ± 0.58 ^a

The data were expressed as mean ± SD; n = 3. The mean values in a column superscripted by the same letter are not significantly different at p ≤ 0.05.