Determination of ethanol in blood using headspace gas chromatography with flameionization detector (HS-GC-FID): Validation of a method

Figures & data

Table 1. Bias data of blood spiked with ethanol and IS at three concentration levels

µo (mg/mL) added	x̅ (mg/mL) found	$\mathit{B}\mathit{i}\mathit{a}\mathit{s}=\left(\bar{\mathbf{x}}-\mu \mathbf{o}\right)$	$\mathit{B}\mathit{i}\mathit{a}\mathit{s}\mathbf{\%}=\left(\frac{\bar{\mathit{x}}-\mu \mathbf{o}}{\mu \mathbf{o}}\right)\ast 100$	$\mathit{R}\mathit{e}\mathit{c}\mathit{o}\mathit{v}\mathit{e}\mathit{r}\mathit{y}\mathbf{\%}=\left(\frac{\bar{\mathbf{x}}}{\mu \mathbf{o}}\right)\ast 100$
0.5	0.45	−0.04	−8.72	91
1.07	1.17	0.10	9.41	109
3.5	3.46	−0.03	−0.86	99

Figure 1. Calibration curve after removal of outlier (2 mg/mL; 0.089416213): RPA(Y) = 0.46*Conc. (mg/mL)- 0.0639

Figure 2. (a) shows chromatogram of Ethanol and IS at the same concentration 0.8 mg/mL and (b) shows spiked human blood containing 0.8 mg/mL methanol, 80 mg/mL acetaldehyde, 0.8 mg/mL ethanol and 0.8 mg/mL IS

Table 2. Statistical evaluation for repeatability and intermediate precision between two analysts

Parameters	Conc. in (mg/mL)		Mean (x̅)	%RSD
Repeatability	0.8 (n = 6) 0.017 27
Intermediate precision	0.8 (n = 3)	Analyst I	0.015	11
	0.8 (n = 3)	Analyst II	0.22	1