OXIDATIVE STRESS-MEDIATED RENAL DYSFUNCTION BY CYCLOSPORINE A IN RATS: ATTENUATION BY TRIMETAZIDINE

Figures & data

Table 1. Effect of Trimetazidine (TMZ) (2.5 mg/kg, p.o) on Body Weight, Water Intake, Urine Output, and Systolic Blood Pressure in CsA (20 mg/kg, s.c.) Treated Rats

Treatment	% Change in Body Weight, gms	Water Intake, mL/24 h	Urine Output, mL/24 h	% Change in Blood Pressure, mm of Hg
Vehicle (Olive oil)	6.72±1.42	31.2±2.82	9.2±1.24	1.99±4.67
CsA (20)	0.37±0.231*	35.0±4.56	9.28±0.87	4.81±6.43
CsA (20) + TMZ (2.5)	3.64±1.12**	35.83±2.40	11.3±0.69	0.98±1.3

All values are expressed as mean±SEM.

*p < 0.05 as compared to control group,

**p < 0.05 as compared to CsA group.

Figure 1. Effect of trimetazidine on CsA-induced lipid peroxidation. Values expressed as mean ± SEM. *p < 0.05 as compared to control group, **p < 0.05 as compared to CsA group. (One-way ANOVA followed by Dunnett's test).

Figure 2. Effect of trimetazidine on plasma creatinine in CsA treated rats. Values expressed as mean ± SEM. *p < 0.05 as compared to control group, **p < 0.05 as compared to CsA group. (One-way ANOVA followed by Dunnett's test).

Figure 3. Effect of trimetazidine on blood urea nitrogen (BUN) in CsA treated rats. Values expressed as mean ± SEM. *p < 0.05 as compared to control group, **p < 0.05 as compared to CsA group. (One-way ANOVA followed by Dunnett's test).

Figure 4. Effect of trimetazidine on creatinine clearance in CsA treated rats. Values expressed as mean ± SEM. *p < 0.05 as compared to control group, **p < 0.05 as compared to CsA group. (One-way ANOVA followed by Dunnett's test).

Figure 5. Effect of trimetazidine on urea clearance in CsA treated rats. Values expressed as mean ± SEM. *p < 0.05 as compared to control group, **p < 0.05 as compared to CsA group. (One-way ANOVA followed by Dunnett's test).

Table 2. Effect of Trimetazidine (TMZ) (2.5 mg/kg, p.o) Administration on Morphological Changes as Assessed by Histopathological Examination of Kidney in CsA (20 mg/kg, s.c.) Treated Rats

Group	Epical Blebbing	Hyaline Casts	Interstitial Vacuolization	Glomerular Basement Membrane Thickening	Arteriol -opathy	Tubulo Interstitial Fibrosis
Control	−	−	−	−	−	−
CsA (20)	+ + +	+ + +	+ +	+ +	+ + +	+ +
CsA (20)+TMZ (2.5)	+	+	−	−	−	−

− None, + Mild, + + Moderate, + + + Severe.

Figure 6. Light microscopic observations of kidney sections of different treatment groups: (A) Renal cortex of a rat treated with vehicle (PAS, X500), (B) Interstitial fibrosis of stripped pattern and tubular atrophy in cortex of CsA treated rat (PAS, X125), (C) Arteriole with marked luminal narrowing and pronounced intimal thickening (Masson's trichome, X500), (D) Renal cortex of a rat treated with CsA (20 mg/kg) and TMZ (2.5 mg/kg) (PAS, X500).