Figures & data
Figure 1. Effects of hypoxia on LDH release in MDCK cells. The cells were cultivated in culture media with glucose and were submitted to hypoxia and the bottles kept sealed for 24 h in the incubator. Data are mean ± SD of culture bottle compared to control group (NS = not significant).
Figure 2. LDH release of MDCK cells deprived of glucose for 24 h and submitted to hypoxia. The cells were washed with PBS and glucose-free culture media was added. Hypoxia was performed and the bottles were immediately sealed to wait 24 h in the incubator. Data are mean ± SD of culture bottle compared to cells deprived glucose only; p<0.05.
Figure 3. LDH release of MDCK cells deprived of glucose for 48 h and submitted to hypoxia. The cells were incubated with glucose-free culture media during 24 h, then the cells were submitted to hypoxia and the bottles were kept sealed for additional 24 h, thereby completing 48 h incubation in a glucose-free culture media, the last 24 h of which were associates with the hypoxia situation. Data are mean ± SD of culture bottle compared to cells deprived glucose only; p<0.05.
Figure 4. Injury protocol.
Figure 5. Effects of growth factors EGF (20 ng/mL), IGF-I (20 ng/mL) or HGF (20 ng/mL) on LDH release in MDCK cells during hypoxic-injury. Data are mean ± SD of culture bottle compared to controls and untreated injury group; *p<0.001 vs. Control; †p<0.001 vs. Injury and EGF; †p<0.05 vs. IGF-I.
Figure 6. Effects of growth factors EGF (20 ng/mL), IGF-I (20 ng/mL) or HGF (20 ng/mL) on viability (acridine orange plus ethidium bromide) in MDCK cells submitted to hypoxic-injury. Data are mean ± SD of culture bottle compared to controls and untreated injury group; *p<0.001 vs. Control; †p<0.01 vs. Injury; †p<0.05 vs. EGF.
Figure 7. Effects of growth factors EGF (20 ng/mL), IGF-I (20 ng/mL) or HGF (20 ng/mL) on morphologic analysis of apoptosis (Hoechst 33342) in MDCK cells submitted to hypoxic injury. Data are mean ± SD of culture bottle compared to controls and untreated injury group; *p<0.001 vs. Control; †p<0.01 vs. Injury; †p<0.05 vs. EGF.
