Early Manifestation of Nephropathy in Rats with Arterial Calcinosis

Figures & data

Table 1. Systolic arterial pressure, total calcium in aorta and plasma, and plasma lipid profile in control and treated rats (vitamin D3 300,000 IU/kg b.w., i.m., 5 days before the experiment). Results are expressed as means±SD

	Control rats (n = 6)	Treated rats (n = 4)
Systolic arterial pressure (mmHg)	97±2	159±5a
Total calcium in aorta (umol/g dry wt)	24±2	38±5a
Total calcium in plasma (mg%)	9.40±0.10	9.20±0.30
Total lipids (g/L)	2.53±0.03	2.54±0.10
Cholesterol (g/L)	0.50±0.02	0.49±0.02
Triglycerides (g/L)	0.48±0.010	0.47±0.13

^ap<0.05.

Figure 1. Renal clearance of SA, renal blood flow and glomerular filtration rate in control and treated rats (vitamin D3 300,000 IU/kg b.w., i.m., 5 days before the experiment). Results are expressed as means ± SEM. (#) p<0.05.

Figure 2. Fractional excretion of water (FE% H2O) and fractional excretion of osmolytes (FE% Osm) in control and treated rats (vitamin D3 300,000 IU/kg b.w., i.m., 5 days before the experiment). Results are expressed as means ± SEM. (#) p<0.05.

Figure 3. Fractional excretion of sodium (FE% Na) and fractional excretion of potassium (FE% K) in control and treated rats (vitamin D3 300,000 IU/kg b.w., i.m., 5 days before the experiment). Results are expressed as means ± SEM. (#) p<0.05.

Figure 4. Total calcium in renal tissue and fractional excretion of calcium (FE% Ca) in control and treated rats (vitamin D3 300,000 IU/kg b.w., i.m., 5 days before the experiment). Results are expressed as means ± SEM. (#) p<0.05.

Figure 5. A: Basolateral membranes (50 ug) from kidneys of control and treated rats were separated on SDS-PAGE. OAT1 was identified using polyclonal antibodies as described in Methods. B: densitometric quantitation of OAT1. Control levels were set at 100%. Each column represents mean ± SEM from experiments carried out in triplicate on 3 different vesicle preparations for each experimental groups. (#) p<0.05.