Urinary Guanidinoacetic Acid Excretion as an Indicator of Gentamicin Nephrotoxicity in Rats

Figures & data

Table 1. Serum Concentrations of BUN, Creatinine, Guanidinoacetic Acid, and Guanidinosuccinic Acid in the Study Rats

	BUN (mg/dL)	Creatinine (mg/dL)	GAA (µg/dL)	GSA (µg/dL)
Control (n = 7)	19.3 ± 1.3	0.71 ± 0.07	85.0 ± 1.7	1.99 ± 0.24
GM 5 mg (n = 7)	19.5 ± 1.1	0.72 ± 0.05	84.6 ± 3.0	2.00 ± 0.19
GM 10 mg (n = 7)	19.6 ± 1.5	0.69 ± 0.08	83.3 ± 4.5	1.99 ± 0.24
GM 30 mg (n = 7)	19.6 ± 1.9	0.80 ± 0.18	80.9 ± 3.9	2.07 ± 0.21

Values are mean ± SE.

BUN: blood urea nitrogen, GAA: guanidinoacetic acid, GSA: guanidinosuccinic acid, GM: gentamicin.

Figure 1. Urinary N‐acetyl‐β‐d‐glucosaminidase activity in rats injected with gentamicin at various doses. NAG: N‐acetyl‐β‐d‐glucosaminidase, GM: gentamicin. ^** p < 0.01.

Figure 2. Urinary γ‐glutamyl transpeptidase and leucine aminopeptidase activity in gentamicin‐treated rats. γ‐GTP: γ‐glutamyl transpeptidase, LAP: leucine aminopeptidase, GM: gentamicin. ^* p < 0.05.

Figure 3. Urinary guanidinoacetic acid excretion in rats injected with gentamicin at various doses. GAA: guanidinoacetic acid, GM: gentamicin. ^** p < 0.01.

Figure 4. Guanidinoacetic acid concentration in the kidney and liver. GM: gentamicin. ^* p < 0.05, ^** p < 0.01.

Figure 5. Renal glycine amidinotransferase activity in gentamicin‐treated rats. GAT: glycine amidinotransferase, GM: gentamicin. ^* p < 0.05, ^** p < 0.01.