Screening Europe 2010: an update about the latest technologies and applications in high-throughput screening

Figures & data

Figure 1. High-throughput screening for compounds controlling self-renewal and differentiation of human embryonic stem cells.

The cells were exposed to a total of 2800 different chemical entities and subsequently stained for Oct4 expression as a marker for the undifferentiated state. Reproduced courtesy of Sabrina Desbordes, Center for Genomic Regulation, Barcelona, Spain.

Figure 2. Screening compounds simultaneously for the inhibition of bacterial growth and cytotoxicity in human cells.

HEK293T cells expressing a fluorescence reporter for cell viability are directly cocultivated with the pathogen of interest (Staphylococcus aureus). (A) In the presence of a noncytotoxic, specific antibiotic (e.g., penicillin/streptomycin), the human reporter cells survive and generate a strong readout signal (inset); whereas, (B) in the absence of a specific antibiotic, the pathogen outgrows the human reporter cells, resulting in shutdown of the readout signal [10].

Figure 3. Whole-organism cytotoxicity assays.

Zebrafish embryos show 85% genetic homology with humans and develop rapidly. This allows the screening of several thousand samples at costs similar to cell-based screens.

Reproduced courtesy of Carlos Callol-Massot, Biobide S.L., San Sebastian, Spain.

Merten CA. Indirect inhibition assays and droplet-based microfluidics. Presented at: 7th Annual Screening Europe Conference. Barcelona, Spain, 11–12 February 2010.

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