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Review

Long-term frozen storage of stem cells: challenges and solutions

Pages 9-20 | Published online: 07 Jul 2016

Figures & data

Figure 1 A typical (cord blood) stem cell collection kit and shipping container.

Notes:The sterile collection pouch containing the collection bag (A) and the necessary equipment needed to collect and ship a cord blood collection (B)
Figure 1 A typical (cord blood) stem cell collection kit and shipping container.

Figure 2 Schematic representation of cord blood processing outcomes.

Abbreviations: DMSO, dimethyl sulfoxide; RBC, red blood cell
Figure 2 Schematic representation of cord blood processing outcomes.

Table 1 Cord blood cell recovery by processing methodology

Figure 3 Long-term viability data for adipose stem cells cryopreserved and banked for 2 years.

Notes:Three independent samples (A, B, and C) are shown in comparison. Samples were collected, processed, banked, and thawed. Viability was determined by Trypan blue dye exclusion after being frozen and thawed for 1 day, which was initially ∼85%–100%. No significant differences were found between any of the time points for a particular sample
Figure 3 Long-term viability data for adipose stem cells cryopreserved and banked for 2 years.

Figure 4 Long-term functional data for CT-MSCs.

Notes:Five independent samples are shown. CT-MSCs were stored for a period of 12 months before thawing and assessment.
Abbreviations: CT-MSC, cord tissue mesenchymal stromal cell
Figure 4 Long-term functional data for CT-MSCs.