Anti-CD30-targeted gold nanoparticles for photothermal therapy of L-428 Hodgkin’s cell

Figures & data

Figure 1 Schematic diagram of the Nd:YAG laser irradiation system.

Abbreviation: ND, neodymium.

Figure 2 Flow cytometry detection of the binding specificity of different gold nanoparticle–antibody conjugates.

Note: The inset box shows the conceptual diagram of L-428 cells stained with gold-BerH2 conjugates and aM-A488.

Abbreviation: Ig, immunoglobulin.

Figure 3 Viability of L-428 cells exposed to either unbound golds or gold-BerH2 conjugates for 6, 12, and 24 hours, as evaluated by flow cytometry.

Notes: Percentage was calculated as the ratio of calcein-AM uptake with L-428 cells divided by the total cells, as calculated for each condition. Control: L-428 cells without gold. Gold (10⁴): L-428 incubated with unbound gold at the ratio of 10⁴:1. Gold-BerH2 (10⁴) and Gold-BerH2 (10⁸): L-428 incubated with gold-BerH2 conjugates at the ratios of 10⁴:1 and 10⁸:1. Error bars represent 1 standard deviation.

Figure 4 Photothermal treatments of L-428 cells with gold-BerH2 conjugates. (A) With 532 nm laser irradiation with 50 mW, 5 pulses; (B) without laser irradiation.

Notes: Cells were stained using calcein-AM and propidium iodide solution, and then analyzed using flow cytometry and a fluorescence microscope. The left side of the figure shows dot plots. The fluorescent images on the right side show calcein-AM (green).

Abbreviations: FSC-H, forward light scatter-height; PI, propidium iodide; SSC-H, side light scatter-height.

Figure 5 Flow cytometry dot plot and statistical analysis diagram of L-428 cells after being irradiated at different laser power settings. (A) L-428 cells; (B) L-428 cells incubated with gold-BerH2 conjugates. (C) The results were analyzed by flow cytometry data.

Notes: *L-428 incubated with gold-BerH2 conjugates; ^#L-428 incubated without gold-BerH2 conjugates. Error bars represent 1 standard deviation.

Abbreviations: mW, microwatts; PI, propidium iodide.

Figure 6 The influence of gold nanoparticles on the viability of L428 cells under four different conjugation conditions: without gold (Control), with unbound golds (Golds), with gold-ACT1 conjugates (Gold-ACT1), and with gold-BerH2 conjugates (Gold-BerH2).

Note: Each test included one group with and one group without laser irradiation.