The SERPINA4 rs2070777 AA Genotype is Associated with an Increased Risk of Recurrent Miscarriage in a Southern Chinese Population

Figures & data

Table 1 Frequency Distribution of Selected Characteristics in Patients with Recurrent Miscarriage and Controls

Variables	Cases (n =631)		Controls (n = 771)		P^a
	No.	%	No.	%
Age range, years	20–46		20–49
Mean ± SD	32.39±5.38		32.50±5.20		0.6978
<35	419	66.4	516	66.75
35–40	155	24.56	187	24.19
>40	57	9.03	70	9.06
No. and % of abortions
2–3	376	59.59
≥4	255	40.41

Notes: ^aThe P value was obtained from a two-sided χ2 test of the distributions between the patients with recurrent miscarriage and the controls.

Table 2 Genotype and Allele Frequencies of SERPINA4 in Patients with Recurrent Miscarriage and Controls

Genotype/Allele	RM (N =631)	Controls (N=771)	P-value	OR (95% CI)	P-value	Adjusted OR (95% CI)	P-value^b
SERPINA4/rs910352 C > T (HWE = 0.173)
CC	194(30.74)	266(34.41)	0.3388	1.00	/	1.00	/
CT	312(49.45)	359(46.44)	/	1.577(1.251–1.988)	0.0001	1.579(1.252–1.992)	0.0001
TT	125(19.81)	148(19.15)	/	1.532(1.140–2.060)	0.0047	1.524(1.134–2.049)	0.0052
Dominant (TT/TC vs CC)	437(69.26)	507(65.59)	0.1449	1.182(0.944–1.480)	0.1455	1.186(0.947–1.485)	0.1382
Recessive (TT vs CT/AA)	506(80.19)	625(80.85)	0.7547	1.043(0.800–1.360)	0.7543	1.039(0.797–1.355)	0.777
SERPINA4//rs2070777 T>A (HWE = 0.226)
TT	233(36.93)	319(41.27)	0.0541	1.00	/	1.00	/
TA	302(47.86)	367(47.48)	/	1.430(1.146–1.785)	0.0016	1.437(1.151–1.794)	0.0014
AA	96(15.21)	87(11.25)	/	1.918(1.376–2.673)	0.0001	1.909(1.369–2.661)	0.0001
Dominant (AA/TA vs TT)	398(63.07)	454(58.73)	0.0972	1.200(0.967–1.490)	0.0977	1.206(0.971–1.497)	0.0896
Recessive (AA vs TA/TT)	535(84.79)	686(88.75)	0.0288	1.415(1.036–1.932)	0.0289	1.409(1.032–1.924)	0.0309
Combined risk-effect of genotypes*
0	346(54.83)	285(45.17)		1	/	1	/
1–2	517(60.19)	342(39.81)	0.0388	1.264(1.025–1.558)	0.0283	1.257(1.019–1.550)	0.0326

Notes: *The risk genotypes used for the calculation were rs910352 CT/TT + rs2070777 AA. Dominant: homozygous rare+heterozygous vs homozygous frequent allele. Recessive: homozygous rare vs heterozygous+homozygous frequent allele. Statistically significant values are shown in bold (P<0.05). ^bAdjusted for age.

Abbreviations: OR, odds ratio; HWE, Hardy–Weinberg equation; RM, recurrent miscarriage.

Table 3 Stratification Analysis of the Associations Between SERPINA4 Polymorphisms and Recurrent Miscarriage Risk in a Southern Chinese Population

Variable	rs2070777 (Cases/Controls)		P	OR (95% CI)	P	Adjust OR (95% CI)	P	Combined (Cases/Controls)*		P	OR (95% CI)	P	Adjusted OR (95% CI)	P
	AT/TT	AA						0	1–2
Age
<35	176/210	31/32	0.5944	1.156 (0.678–1.970)	0.5942	/	/	116/128	91/115	0.4752	0.873 (0.602–1.267)	0.4754	/	/
35–40	213/280	40/37	0.1523	1.421(0.878–2.300)	0.1523	/	/	134/237	119/134	0.0065	1.571(1.135–2.175)	0.0065	/	/
>40	146/196	25/18	0.0555	1.865 (0.981–3.545)	0.0574	/	/	96/152	75/93	0.228	1.277 (0.858–1.900)	0.2279	/	/
No. of abortions
2–3	313/686	63/87	0.0107	1.587(1.118–2.254)	0.0098	1.548 (1.088–2.201)	0.0151	210/517	166/342	0.155	1.195(0.935–1.527)	0.1544	1.161 (0.907–1.486)	0.2356
≥4	222/686	33/87	0.4715	1.172(0.764–1.799)	0.4674	1.177(0.766–1.810)	0.4575	136/517	119/342	0.0519	1.323 (0.998–1.753)	0.0514	1.314 (0.991–1.742)	0.058

Notes: *The combination of risk genotypes used for the calculation was SERPINA4 rs910352 CT/TT + rs2070777 TT. Statistically significant values are shown in bold (P<0.05).