Figures & data
Table 1 Anthropometric data and data from the health questionnaire
Figure 1 OPLS-DA model of quantified proteins from plasma.
Notes: (A) A score plot of CWP compared to CON, visualizing clear separation between the 2 included groups. (B) Loading plot corresponding to significant proteins, with a VIP value >1 (triangles), between CWP and CON. The parameters for the models were R2=0.84, Q2=0.60, CV-ANOVA, p-value=2.31e−06
Abbreviations: OPLS-DA, orthogonal partial least square discriminant analysis; CWP, chronic widespread pain; CON, controls; VIP, variable influence of projection; CV-ANOVA, cross-validated analysis of variance.
Abbreviations: OPLS-DA, orthogonal partial least square discriminant analysis; CWP, chronic widespread pain; CON, controls; VIP, variable influence of projection; CV-ANOVA, cross-validated analysis of variance.
Figure 2 A representative SYPRO Ruby® stained 2-DE gel of the plasma proteome from one woman with CWP (right) and one healthy CON (left).
Notes: A total of 22 proteins (marked, numbered spots), including different isoforms, had a VIP value >1, were highly significant for the model, and were further identified.
Abbreviations: 2-DE, 2-dimensional gel electrophoresis; CWP, chronic widespread pain; CON, controls; VIP, variable influence of projection; MW, molecular weight; pI, isoelectric point.
Abbreviations: 2-DE, 2-dimensional gel electrophoresis; CWP, chronic widespread pain; CON, controls; VIP, variable influence of projection; MW, molecular weight; pI, isoelectric point.
Table 2 Identified proteins in plasma
Figure 3 Concentration of VDBP in plasma for the 2 groups of subjects.
Note: Significantly elevated concentration of VDBP was found in CWP compared to CON (*p=0.0343).
Abbreviations: VDBP, vitamin D-binding protein; CWP, chronic widespread pain; CON, controls.
Abbreviations: VDBP, vitamin D-binding protein; CWP, chronic widespread pain; CON, controls.
Table S1 Mass spectrometry and MVDA data from identified proteins with a VIP>1