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Original Research

Bio Micro-Nano Technologies of Antioxidants Optimised Their Pharmacological and Cellular Effects, ex vivo, in Pancreatic β-Cells

ORCID Icon, , ORCID Icon, , ORCID Icon, , & ORCID Icon show all
Pages 1-9 | Published online: 07 Jan 2020

Figures & data

Figure 1 SEM images of microcapsule F1–F6 (A), FTIR-chromatograms (B), thermal peaks (C), and electrokinetic voltage (D). Data are mean ± SEM, n=3. F2, F4 and F6 are formulation matrices with UDCA, probucol and addition of NM30D, RL30D or RS30D polymers, respectively. F1, F3 and F5 are corresponding controls without UDCA.

Figure 1 SEM images of microcapsule F1–F6 (A), FTIR-chromatograms (B), thermal peaks (C), and electrokinetic voltage (D). Data are mean ± SEM, n=3. F2, F4 and F6 are formulation matrices with UDCA, probucol and addition of NM30D, RL30D or RS30D polymers, respectively. F1, F3 and F5 are corresponding controls without UDCA.

Figure 2 Mechanical stability (A), osmotic stability (B) and probucol release profile from the microcapsules at pH 1.5 and 7.4. Data are mean ± SEM, n=3. **p<1%.

Figure 2 Mechanical stability (A), osmotic stability (B) and probucol release profile from the microcapsules at pH 1.5 and 7.4. Data are mean ± SEM, n=3. **p<1%.

Figure 3 Pancreatic β-cells uptake of probucol release from the microcapsules at glucose 5.5 mM and 35.5 mM. Data are mean ± SEM, n=3. **p<1%.

Figure 3 Pancreatic β-cells uptake of probucol release from the microcapsules at glucose 5.5 mM and 35.5 mM. Data are mean ± SEM, n=3. **p<1%.

Figure 4 Viability of NIT-1 cells after exposure to the microcapsules, and corresponding fluorescence (cellular oxidative stress) levels. Each treatment group (F2, F4 or F6) was compared with its corresponding control (F1, F3 or F5). Data are mean ± SEM, n=3. *p-value <0.05, ** p-value <1%.

Figure 4 Viability of NIT-1 cells after exposure to the microcapsules, and corresponding fluorescence (cellular oxidative stress) levels. Each treatment group (F2, F4 or F6) was compared with its corresponding control (F1, F3 or F5). Data are mean ± SEM, n=3. *p-value <0.05, ** p-value <1%.