Epigallocatechin-3-gallate suppresses cell proliferation and promotes apoptosis and autophagy in oral cancer SSC-4 cells

Figures & data

Figure 1 Time-dependent cell proliferation evaluation for EGCG treatment at different EGCG doses (0, 10, 20, 50, 100, and 200 μM), in SSC-4 cells, using the xCELLigence system.

Notes: (A) Data are displayed as the normalized cell index, over 49 hours. (B) Time-dependent IC₅₀ evaluation using RTCA software.
Abbreviations: EGCG, Epigallocatechin-3-gallate; IC₅₀, half maximal inhibitory concentration; RTCA, real-time cell analysis.

Figure 2 Flow cytometry evaluation using annexin V FITC/PI, showing the effects of 20 μM EGCG treatment for 24 hours and 48 hours on SSC-4 cells.

Notes: Viable cells are located in the lower left quadrant, and early apoptotic cells are positioned in the lower right quadrant of the two flow cytometric displays, while late apoptotic cells are located in the upper right quadrant, and necrotic cells are displayed in the upper left quadrant (24 hours). The image at the right presents the % apoptotic, necrotic, and viable cells, from total numbers of cells.
Abbreviations: EGCG, epigallocatechin-3-gallate; FITC, fluorescein isothiocyanate; PI, propidium iodide.

Figure 3 EGCG-induced autophagy in SSC-4 cells, using fluorescence microscopy evaluation after MDC staining.

Notes: (A) The control shows a low level of basal autophagy, indicated by blue “dot” staining of the autophagic vacuole, while MDC staining of SSC-4 cells treated with 20 μM EGCG led to an increase in fluorescence intensity and number of autophagic vacuoles compared with the untreated cells. (B) Bright-field microscopy. (C) Evaluation of the MDC fluorescence intensity, using a BioTek Microplate Reader.
Abbreviations: EGCG, epigallocatechin-3-gallate; MDC, monodansylcadaverine.

Figure 4 Relative gene expression level showing the effect of treatment with a single dose of EGCG (20 μM), at 24 hours posttreatment.

Abbreviation: EGCG, epigallocatechin-3-gallate.

Figure 5 Gene network, created using IPA^® software, reflecting EGCG treatment.

Notes: The overexpressed genes are labeled in red, while those downregulated are marked in green.
Abbreviations: EGCG, epigallocatechin-3-gallate; IPA, Ingenuity^® Pathway Analysis.

Figure 6 Putative model of multiple antitumoral mechanisms activated by EGCG, in SSC-4 human oral cancer cells.

Notes: Overexpressed genes are labeled in red, and downregulated in green.
Abbreviation: EGCG, epigallocatechin-3-gallate.

Figure 7 p53, FAS, and β-actin immunoblotting on SSC-4 cells, showing the effects of treatment with a single dose of EGCG (20 μM), at 48 hours posttreatment.

Abbreviation: EGCG, epigallocatechin-3-gallate.