Figures & data
Figure 1. Temperature profile in time measured with a fiber optic probe at three positions in mouse leg (intradermal, subcutaneously and intramuscular) during warm water bath heating at three different temperatures.
Figure 2. Bioluminescence image taken 4 hours after heating the right leg of NLF-1 mouse in a water bath. The colors show the light intensity measured with an optical camera from low intensity (violet) to high intensity (red). The position of the ROI used for quantification of the bioluminescence signal is also indicated.
Figure 3. Time course of luciferase activity after heating a mouse leg with different heating protocols (A) 4, 8 and 16 min at 43°C, (B) 2, 4 and 8 min at 44°C, and (C) 1, 2 and 4 min at 45°C. The signal intensity (SI) is the average light intensity measured in the ROI, which is indicated in figure 2. The control measurement is done at the same position in the non-heated leg of the mice that were heated for the longest duration at each temperature.
Figure 4. Time course of luciferase activity after heating a mouse leg. The data from figure 3 are rearranged in such a way that the luciferase activity is shown for constant heating duration (4 min) at 3 different temperatures. The signal intensity (SI) is the average light intensity measured in the ROI, which is indicated in figure 2. The control measurement is done at the same position in the non-heated leg of the mice that were heated at 45°C.
Figure 5. Bioluminescence image taken 4 hours after heating the left leg of NLF-1 mice for 16 min at 43°C (A, C) or 4 min at 45°C (B, D) in a water bath. In figures C and D the skin is locally removed (see inserts) to show the signal coming from the muscle. The numbers in figure indicate the average signal intensity (counts per second) measured in the ROIs as indicated in the figures. The colors show the light intensity measured with an optical camera from low intensity (violet) to high intensity (red).
Figure 6. Arrhenius plot for constant-temperature in vivo water bath experiments 4 hours after heating. The error bars represent the standard deviation of the averages for each temperature. The solid line is a linear fit with error weighting performed on three data points, corresponding to 43, 44 and 45°C.
Figure 7. Arrhenius plot for constant-temperature ex vivo hyperthermia experiments 5 hours after heating. The solid line is a linear fit with error weighting performed on three data points, corresponding to 43, 44 and 45°C for the MEF. The dashed line is a linear fit with error weighting performed on three data points for BMM cells. The error bars represent the standard deviation of the averages for each temperature.
