Clinical and immunologic results of local hyperthermia at 44 °C for extensive genital warts in patients with diabetes mellitus

Figures & data

Figure 1. Extensive genital warts in patients with type I DM. Warty lesions before treatment (A), four weeks after hyperthermia (B), complete clearance of lesions nine weeks after hyperthermia (C).

Figure 2. Immunohistochemical staining for CD4, CD8, CD1a and CD68 cells in untreated lesions distant from the target lesions (10×).Thumbnails were taken from the same picture (40×).

Table I.  Counts of infiltrating immune cells in lesions before and 2, 4, 6 weeks after local hyperthermia in one patient. CD1a positive cells were distributed in both the epidermis and dermis, whereas CD4+, CD8+ and CD68+ cells were mostly distributed in the upper dermis close to the epidermis. The numbers of positive staining cells were counted under an eyepiece graticule with a high-power (×40) objective lens. Counts were done in at least 10 representative fields and cell numbers were expressed as mean ± SD.

	Pretreatment		Post-treatment (weeks)
	0	2	4	6
CD4 (dermis)	120.70 ± 28.23	193.40 ± 36.75**	214.80 ± 28.98**	135.60 ± 18.47
CD8 (dermis)	49.00 ± 21.71	101.70 ± 53.27**	100.40 ± 32.03**	69.60 ± 22.05
CD1a (dermis)	12.80 ± 10.01	16.90 ± 10.08	26.60 ± 15.99	27.00 ± 14.06*
CD1a (epidermis)	41.40 ± 15.00	27.20 ± 5.88	54.60 ± 16.08	76.00 ± 17.07**
CD68 (dermis)	33.50 ± 8.61	43.50 ± 10.89	60.20 ± 22.60**	31.60 ± 6.45

Dunnett's t test from the statistical package of one-way ANOVA was used to compare the number differences of infiltrating cells between the pretreatment (0 week) and those at 2 weeks/4 weeks/6 weeks, respectively. A p value of <0.05 was regarded as statistically significant (**P < 0.01; *P < 0.05).