Figures & data
Figure 1. SCK tumours were treated in vivo with (A) ICG at 4 mg/kg followed by laser thermal therapy or (B) local hyperthermia at 42.5 °C before ICG administration at 4 mg/kg followed by laser thermal therapy. Near-infrared imaging was performed for 30 min following therapy with snapshots taken every 60 s to assess fluorescent intensity of ICG in the tumour.
Figure 2. Hyperthermia increases fluorescent intensity of ICG in SCK tumours. Hyperthermia was administered at 42.5 °C for 60 min prior to ICG (4 mg/kg bodyweight) administration and whole body near-infrared imaging. Each treatment group consisted of five animals.
Figure 3. Hyperthermia increases fluorescent intensity of ICG in SCCVII tumours. Hyperthermia was administered at 42.5 °C for 60–90 min prior to ICG (4 mg/kg bodyweight) administration and whole body infrared imaging.
Figure 4. Mean SCK tumour volume as a function of time (days) after treatment. Tumours were treated with hyperthermia for 60 min prior to ICG administration. Thereafter, one laser pulse was delivered to the tumour region. Tumour diameters were measured every day and tumour volume was calculated by the formula a2b/2, where a and b are the shorter and longer diameters of the tumour, respectively. Measurements were taken every 24 h.
Figure 5. Mean SCCVII tumour volume as a function of time (days) after treatment. Tumours were treated with hyperthermia for 60 min prior to ICG administration. Thereafter, one laser pulse was delivered to the tumour region. Tumour diameters were measured every day and tumour volume was calculated by the formula a2b/2, where a and b are the shorter and longer diameters of the tumour, respectively. Measurements were taken every 48 h.
Figure 6. H&E stain after treatment. Tumours were harvested 72 h post-treatment and subsequently treated with H&E stain, fixed and mounted. (A) SCK tumours were treated with ICG and laser. (B) SCK tumours were treated with hyperthermia at 42.5 °C for 60 min, ICG and laser. (C) SCK tumours were treated with ICG and laser. The portion of this slide that has been outlined in red denotes the tumour tissue. Within this region the white area is dead while the black portion is live tissue. (D) SCK tumours were treated with hyperthermia at 42.5 °C for 60 min, ICG and laser. The portion of this slide that has been outlined in red denotes the tumour tissue. Within this region the white area is dead while the black portion is live tissue.
