Figures & data
Figure 1. This figure represents the number of post-treatment days required for the tumours to reach three times treatment volume. mNP + AMF + cisplatin was 1.7 times more effective than mNP + AMF (36 versus 21 days), 1.4 times more than cisplatin + AMF (36 versus 25 days) and 2.6 times more than no treatment (36 versus 14 days). *p values less than 0.003 when compared to mNP + cisplatin + AMF; **p values less than 0.02 when compared to no treatment.
Table I. Summary of tumour and core temperatures of mice that received mNP + AMF or mNP + AMF + CDDP. The majority of tumours achieved CEM60 in the centre of the tumour. Total hyperthermia dose was delivered in less than 20 min. Three mice that received mNP only (1) and mNP + AMF + CDDP (2) had a reduced heating kinetic. Ten tumours achieved CEM60 in less than 20 min (average treatment was 15.3 min). Three tumours required more than 20 min (average treatment time = 51.6 min). In spite of this issue there was no difference for these groups with respect to treatment efficacy (tumour regrowth delay).
Table II. Experimental groups, treatment summary and efficacy.
Figure 2. Low (10×, left) and high (100×, right) magnification photomicrographs of a MTGB female mouse mammary adenocarcinoma accessed 24 h following systemically administered cisplatin at 5 mg/kg body weight. Although some tumour cells remain morphologically normal, the majority of the cells have a reduced volume and both apoptotic and necrotic cells are present. H&E stain.
Figure 3. Low magnification (2×, upper left) photomicrograph of a bi-lobed MTGB flank mammary adenocarcinoma treated with mNPH (CEM60) to the centre of the tumour 24 h following treatment. Regions ‘A’ and ‘B’, represented correspondingly by high magnification photomicrographs, demonstrate uniform tumour necrosis. The high magnification photograph of region ‘C’ demonstrates individual cell damage but also significant tumour viability. Regions ‘A’and ‘B’ contained significant mNP/Fe, while region ‘C’ did not.
Figure 4. The low magnification (2×) photomicrograph of a MTGB flank mammary adenocarcinoma (top) treated with mNPH (CEM60 to the centre of the tumour, 24 h after treatment) and cisplatin demonstrated uniform tumour necrosis. Higher magnification (100×) demonstrates a more detailed view of the necrosis (A) and the partially viable tumour present in isolated regions (B). The regions with viable tumour are located primarily at the deep tumour margin. This tumour was assessed 24 h following treatment, therefore additional treatment effect is likely ongoing. A normal hyperplastic lymph node is visible in the lower right quadrant of the low magnification image. H&E stain.
