Role of iron oxide core of polymeric nanoparticles in the thermosensitivity of colon cancer cell line HT-29

Figures & data

Table 1. Particle size and Zeta potential of nanoparticles.

Type of nanoparticles	Hydrodynamic particle size (nm)	Polydispersity index (PdI)	Zeta potential ± SD
5-Fluorouracil-loaded PLGA-coated nanoparticles with iron core	40.6	0.385	−22.51 ± 1.62
PLGA-coated nanoparticles with iron core	61.4	0.221	−20.82 ± 1.16
5-Fluorouracil-loaded PLGA-coated nanoparticles without iron core	36.2	0.208	−27.12 ± 0.29
PLGA-coated nanoparticles without iron core	83.9	0.253	−15.44 ± 1.33

Figure 1. Transmission electron micrographs of nanoparticles: (A) 5-FU-loaded PLGA-coated nanoparticles with iron core, (B) PLGA-coated nanoparticles without iron core.

Figure 2. In vitro release profiles of 5-FU from 5-FU-loaded PLGA-coated nanoparticles with/without iron core. The plot represents mean ± SD of triplicate results.

Figure 3. The images confirm the entry of nanoparticles into the cell: (A) control image, (B) image with nanoparticles.

Figure 4. Effects of different concentrations of 5-FU and hyperthermia on (A) 5-FU-loaded PLGA-coated nanoparticles without iron oxide core, (B) 5-FU-loaded PLGA-coated nanoparticles with iron core with hyperthermia, and (C) the viability of HT-29 spheroid culture cells. The viability was measured using trypan blue dye exclusion assay method. Mean ± SEM of three experiments.

Figure 5. Effects of different concentrations of 5-FU with and without hyperthermia on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.

Figure 6. Effects of different concentrations of 5-FU or 5-FU-loaded nanoparticles with and without iron core on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.

Figure 7. Effects of 5-FU-loaded PLGA-coated iron-free nanoparticles with and without hyperthermia on induced DNA damages of HT-29 spheroid culture cells. Mean ± SEM of three experiments.

Figure 8. Effects of 5-FU-loaded PLGA-coated iron oxide nanoparticles with and without hyperthermia on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.

Figure 9. Effects of 5-FU-loaded nanoparticles with and without iron oxide combined with hyperthermia on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.