Figures & data
Figure 1. Changes of body temperature (°C) over time (h) of rats treated i.p. with PSP (100 mg kg−1) or 0.9% sterile saline at 7:00 a.m. (black arrowhead) and then injected i.p. with LPS (50 µg kg−1) or 0.9% sterile saline at 9:00 a.m. (white arrowhead) in comparison to non-treated animals (NT). Values are means ± SEM at 30-min averages. n, sample size in a respective group, *significant differences between PSP/LPS and saline/LPS groups; #significant differences between examined groups (PSP/LPS and saline/LPS) and control groups (NT and PSP/saline) at defined time intervals (**p < 0.01; ###p < 0.001, respectively).
Figure 2. Plasma levels of IL-6 (pg mL−1) estimated at 3 h and 14 h post-injection of PSP or saline in the rats injected i.p. with PSP (100 mg kg−1) or saline 2 h prior to the LPS administration (50 µg kg−1) in comparison to non-treated animals (NT) and rats pre-treated with PSP followed by sterile saline. Values are expressed as means ± SEM. Assays were performed on four individuals in each group. *significant difference (***p < 0.001).
Figure 3. Plasma levels of TNF-α (pg mL−1) estimated at 3 h post-injection of PSP or saline in the rats injected i.p. with PSP (100 mg kg−1) or saline 2 h prior to the LPS administration (50 µg kg−1) in comparison to non-treated animals (NT) and rats pre-treated with PSP followed by sterile saline. Values are expressed as means ± SEM. Assays were performed on four individuals in each group. *significant difference (**p < 0.01 and ***p < 0.001, respectively).
Figure 4. Changes of body temperature (°C) over time (h) of rats treated i.p. with PSP (100 mg kg−1) or 0.9% sterile saline at 7:00 a.m. (black arrowhead), then injected i.p. with LPS (50 µg kg−1) or 0.9% sterile saline at 9:00 a.m. (white arrowhead) and finally administered i.p. with rabbit polyclonal IgG anti rat IL-6 or rabbit IgG at 17:00 p.m. (30 µg/rat, grey arrowhead). Values are means ± SEM at 30-min averages. n, sample size in a respective group; *significant differences between PSP/LPS + IgG and PSP/LPS + anti-IL-6 groups; #significant differences between examined groups of rats and non-treated animals (NT) at defined time intervals (**p < 0.01; ##p < 0.01; ###p < 0.001, respectively).
Figure 5. Changes of body temperature (°C) over time (h) of rats injected i.p. with sterile 0.9% saline at 7:00 a.m. (control vehicle for PSP injection, black arrowhead) and at 9:00 a.m. (control vehicle for LPS administration, white arrowhead), and finally treated i.p. with rabbit polyclonal IgG anti-rat IL-6 or rabbit IgG at 17:00 (30 µg/rat, grey arrowhead) in comparison to non-treated animals (NT). Values are means ± SEM at 30-min averages. n, sample size in a respective group.
