A study of the expression of Cyclin E and its isoforms in tumor and adjacent mucosa, correlated to patient outcome in early colon cancer

Figures & data

Figure 1. The place and role of Cyclin E in the cell cycle.

Figure 2. Cyklin E expression in tumor and mucosa as both FL-form (50kDa) and LMW-isoform (35kDa). Examples of expression strength scoring is also shown.

Table I. Patient demography and pathology data.

Age (median(range)) years	71 (50–80)
Gender (male/female)	57/57
Acute/Elective resection	1/113
Location in colon (right/left)	60/54
Hospital stay (median(range)) days	6 (3–44)
Adenocarcinoma	114/114
Cancer stage (UICC/AJCC) stage I	23
stage II	91
Assessed lymph nodes (median(range))	17 (6–59)
Differentiation grade (well/medium/poor/undiff.)	7/87/16/4

Table II. The expression of cyclin E in colon and the association between FL and LMW isoform expression (n(%)) in adjacent mucosa and tumour in stage I/II colon cancer (n=114).

		Expression of full length form
Expression of LMW isoform		None (0)	Weak (1)	Strong (2)	Total
Cyclin E in adjacent mucosa	None (0)	47 (41.2)	0 (0)	0 (0)	47 (41.2)
	Weak (1)	52 (45.6)	1 (0.9)	0 (0)	53 (46.5)
	Strong (2)	12 (10.5)	1 (0.9)	1 (0.9)	14 (12.3)
	Total	111 (97.3)	2 (1.8)	1 (0.9)	114 (100)
Cyclin E in tumour	None (0)	30 (26.3)	2 (1.8)	2 (1.8)	34 (29.9)
	Weak (1)	44 (38.6)	8 (7.0)	4 (3.5)	56 (49.1)
	Strong (2)	11 (9.6)	4 (3.5)	9 (7.9)	24 (21.0)
	Total	85 (74.5)	14 (12.3)	15 (13.2)	114 (100)

Table III. The association between total expression in tumour and total expression in adjacent mucosa of Cyclin E in both full length (FL) form and in the LMW-isoform (n(%)) in stage I/II colon cancer (n=114).

		Total expression in tumour
Cyclin E Total (FL+LMW)		0	1–2	3–4	Total
Total expression in mucosa	0	15 (13.2)	28 (24.5)	3 (2.6)	46 (40.3)
	1–2	15 (13.2)	38 (33.3)	13 (11.4)	66 (57.9)
	3–4	1 (0.9)	1 (0.9)	0 (0)	2 (1.8)
	Total	31 (27.3)	67 (58.7)	16 (14.0)	n=114

Figure 3. Cancerspecific survival in stage III colon cancer, grouped by total cyclin E expression in the tumor (1-low expression. 4-high total expression).

Table IV. Demography and pathology data by the total Cyclin E expression in the tumour

Cyclin E expression tumour (fl+lmw total)	n	Diff grade(Well/medium/poor/undiff)	Cancer stage z(I/II)	T stage(1–4)	Assessed lymph nodes(median)	Risk recurrence (Recur/total/%)	Risk multiple location recurrence (Recur/total)
0	30	2/20/5/3	7/23	0/7/22/1	18	3/30 (10.0%)	0/30
1	46	1/39/6/0	6/40	2/4/39/1	17	0/46 (0%)	0/46
2	21	2/16/2/1	4/16	0/4/16/1	17	1/19 (5.3%)	0/19
3	8	1/6/1/0	3/5	0/3/4/1	17	0/8 (0%)	0/8
4	9	1/6/2/0	3/6	0/3/6/0	19	4/9 (44.4%)	3/9
Total	114	7/87/16/4	23/91	2/21/87/4	17	8/114 (7.0%)	3/114