Matrix metalloproteinase (MMP)-7 in salivary gland cancer

Figures & data

Figure 1. Immunohistochemical stainings of MMP-7 in salivary gland cancer. The intensity of the cytoplasmic staining on a scale from 0 to 3 and the percentage of staining cells in parentheses. (A) Healthy salivary gland tissue. The intensity of epithelial cells stained is 1, some of the acinar cells are positive; (B) AdCC (3, 95%). Staining of nuclei is also visible; (C) AcCC (1, 40%). Some nuclei are stained; (D) MEC (2, 30%); (E) SDC (1, 30%). Scale bars: A, B, C, D, 100 μm.

Table I. Immunohistochemical evaluation of MMP-7 in salivary gland cancer tissue. The intensity is evaluated on a scale of 0-3. Percent is the proportion of staining cells. The index is the product of staining intensity and percentage of positive cancer cells.

			Intensity		%		Index
	Histological type	N	Mean (±SD)	Median	Mean (±SD)	Median	Mean (±SD)	Median
MMP-7
	all	107	1.5 (0.8)	2.0	44.3 (28.8)	40	75.6 (59.0)	60
	AdCC	47	1.7 (0.9)	2.0	42.8 (24.7)	40	86.6 (63.3)	80
	AcCC	24	1.3 (0.8)	1.5	34.6 (29.4)	35	55.2 (52.5)	40
	MEC	23	1.3 (0.7)	1.0	57.4 (33.0)	70	81.5 (56.4)	80
	SDC	13	1.2 (0.6)	1.0	44.2 (28.3)	40	62.7 (52.1)	50

AdCC = adenoid cystic carcinoma; AcCC = acinic cell carcinoma; MEC = mucoepidermoid carcinoma; SDC = salivary duct carcinoma

Table II. Age-adjusted Cox regression analysis of MMP-7 immunohistochemistry and overall survival of patients with salivary gland carcinoma.

	Age-adjusted analysis (n = 107)
Variable	P-value	Hazard ratio (95% Cl)
MMP-7 staining intensity (2–3 vs. 0–1)	0.15	0.69 (0.42–1.14)
MMP-7 percent of staining cells	0.36*
2 vs. 1	0.19	1.49 (0.82–2.69)
3 vs. 1	0.83	1.08 (0.52–2.25)
MMP-7 intensity × %	0.19*
2 vs. 1	0.08	1.74 (0.93–3.28)
3 vs. 1	0.60	1.22 (0.58–2.57)

Table III. Age-adjusted and multivariate Cox regression analysis of prognostic factors for overall survival of patients with in salivary gland carcinomas.

	Age-adjusted analysis (n = 101 – 107)		Multivariate analysis (n = 101)
Variable	P-value	Hazard ratio (95% Cl)	P-value	Hazard ratio (95% Cl)
Age * (n = 107)			<0.001	1.05 (1.03–1.07)
T class (2–4 vs. 1) (n = 103)	0.004	2.46 (1.34–4.53)	0.941	0.97 (0.48–1.99)
N class (N1–N3 vs. N0) (n = 101)	<0.001	3.67 (2.03–6.62)	0.174	1.89 (0.76–4.73)
Stage (II–IV vs. I) (n = 103)	<0.001	5.11 (2.89–9.02)	<0.001	6.59 (2.82–15.44)
Gender (male vs. female) (n = 107)	<0.001	2.64 (1.58–4.41)	0.0009	2.86(1.54–5.32)
Histopathological diagnosis (n = 107)	0.082**		0.018**
Adenoid cystic carcinoma vs. acinic cell carcinoma	0.019	2.70 (1.18–6.18)	0.012	3.12(1.29–7.56)
Salivary duct carcinoma vs. acinic cell carcinoma	0.042	2.85 (1.04–7.82)	0.755	0.84 (0.27–2.56)
Mucoepidermoid carcinoma vs. acinic cell carcinoma	0.318	1.64 (0.62–4.34)	0.070	2.55(0.93–7.03)
VCI Ki-67*** (> 20 vs. ≤ 20)	<0.0001	3.45(1.97–6.04)	0.0036	2.55(1.36–4.80)

*Age was analyzed as a continuous variable

**Overall p-value

***Volume corrected index of Ki-67, data from [15]

Luukkaa H, Klemi P, Leivo I, Vahlberg T, Grenman R. Prognostic significance of Ki-67 and p53 as tumor markers in salivary gland malignancies in Finland: An evaluation of 212 cases. Acta Oncol 2006;45:669–75.

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