Figures & data
Figure 1. Down-regulation of IGF-1R mRNA in colon cancer cells following transient transfection with IGF-1R specific siRNA. Total RNA was extracted at the indicated times after transfection and IGF-1R mRNA was quantitated by semi-quantitative RT-PCR. Shown are the relative mRNA levels of IGF-1R in reference to β-actin expression. Data are expressed as the mean ± standard deviation.
Figure 2. Expression of IGF-1R protein in SW480 cells. Transfection of pkD-shRNA-IGF-1R-V2 results in greatest knockdown at 48 hours, but still provides an 81.2±1.8% knockdown after four days in comparison with midi ones of control cells (untreated SW480 cells, SW480 cells transfected ones with FuGENE HD Transfection Reagent only and cells transfected with PkD-shRNA-NegCon-V1). Data are expressed as the mean ± standard deviation.
Figure 3. Effect of combined treatment (IGF-1R siRNA and irradiation) on viability of SW480 cells. Cell viability of all four groups of SW480 cells including untreated SW480 cells, FUGENE HD Transfection Reagent, pkD-shRNA-NegCon-V1 as cell controls for siRNA and pkD-shRNA-IGF-1R. Also this entire four cell groups used as cell controls for ionizing radiation (curve A) for irradiation of the same treatment cells in 24 h (B), 48 h (C) and 72 h (D). SW480 cells were seeded in 96-well culture plates (4 000 cells/well) and treated with IGF-1R siRNA, irradiated with 1, 5, 10 and 20 Gy or irradiation combined with IGF-1R siRNA treatment. Results are expressed as relative growth ratios of control cells. Each bar represents the relative cell growth inhibition (mean ± standard deviations) from three independent experiments. *p < 0.01, compared to untransfected and transfected cells with pkD-shRNA-IGF-1R-V2 before irradiation.
