Conditionally replicating adenoviruses carrying mda-7/IL-24 for cancer therapy

Figures & data

Figure 1. A schematic diagram of the anti-tumor effects of CRAds-IL-24 therapy. (A) When normal cells are infected with CRAds-IL-24, neither transgene expression nor apoptosis will occur due to the inability of CRAds to replicate in the normal cells.(B) However, when tumor cells are infected with CRAds-IL-24, conditional replication of CRAds within infected tumor cells produces thousands of viral copies. In addition, the IL-24 gene can frequently be expressed at high levels and transported to the cytoplasm. The proapoptotic effects of IL-24 and viral replications lead to tumor cell lysis. Released viral particles spread and infect adjacent tumor cells, eventually leading to tumor regression or even eradication.

Table I. Targeting gene-virotherapy using CRAds armed with the therapeutic gene IL-24.

CRAds-IL-24	Modification	Tumor targeted	Studies	Reference
Ad.PEG-E1A-mda-7	the progression elevated gene-3 (PEG-3) promoter	Prostate cancer, breast cancer, melanoma	in vitro/in vivo	[32,34,35]
ZD55-IL-24	55kDa deletion in the E1B gene	*Pan	in vitro/in vivo	[37,38]
MUD55-IL-24	55kDa deletion in the E1B gene, the MUC1 promoter	Gastric cancer	in vitro/in vivo	[43]
Ad.DD3-E1A-IL-24	the minimal DD3 promoter	Prostate cancer	in vitro/in vivo	[47]
Ad.sp-E1A(Δ24)-IL-24	24-bp deletion in the E1A gene, the survivin promoter	*Pan	in vitro/in vivo	[50]
Ad.sp-E1A(Δ24)-E1B(Δ55)-IL-24	24-bp deletion in the E1A gene,	*Pan	in vitro/in vivo	[51]
	55kDa deletion in the E1B gene, the survivin promoter
AdCN205-IL-24	Deleted 6.7K/gp19Kof E3 genes	Hepatocellular carcinoma, colorectal carcinoma	in vitro/in vivo	[56]

*“Pan” stand for a panel of human cancer cells.

Table II. CRAds armed with IL-24 in combination with chemotherapeutics.

CRAds-IL-24	Chemotherapeutics	Tumor targeted	Effect	Studies	Reference
ZD55-IL-24	Dacarbazine	Melanoma	Additive or synergistic	in vitro	[57]
ZD55-IL-24	Cisplatin	*Pan	Enhanced	in vitro	[59]
ZD55-IL-24	Adriamycin or Cisplatin	Lung	Enhanced	in vitro/in vivo	[58]
ZD55-IL-24	Dichloroacetate	*Pan	Enhanced	in vitro	[61]
ZD55-IL-24	Wortmannin	Leukemia	Enhanced	in vitro/in vivo	[62]
CRAdRGDflt-IL24	Temozolomide	Glioma	Enhanced	in vitro/in vivo	[69]

*“Pan” stand for a panel of human cancer cells.

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