Cyclin E1 is a strong prognostic marker for death from lymph node negative breast cancer. A population-based case-control study

Figures & data

Table I. Characteristics of our patients and their breast tumours.

Parameter	Case n (%)	Control n (%)
Average age (years)	66	61
Tumour histology
Ductal	160 (86)	144 (77)
Lobular	19 (10)	22 (12)
Others	7 (4)	20 (11)
Histological grade
1	17 (9)	46 (25)
2	93 (47)	103 (55)
3	75 (40)	34 (18)
Not known	1 (0)	3 (1)
Average tumour size	20 mm	16 mm
ER status
Positive	102 (55)	145 (78)
Negative	78 (42)	39 (21)
Not known	6 (3)	2 (1)
PgR status
Positive	72 (39)	126 (68)
Negative	107 (58)	58 (31)
Not known	7 (4)	2 (1)
HER 2 status
Overexpression (IHC3 + or FISH pos.)	18 (10)	13 (7)
Normal	157 (84)	158 (85)
Not known	11 (6)	15 (8)

FISH, fluorescent in situ hybridization; IHC, immunohistochemistry.

Image 1. High expression of cyclin E.

Image 2. Low expression of cyclin E.

Table II. The correlation between cyclin E1 and tumour characteristics.

Cyclin E1	r E1200	p-value E1200	r E1500	p-value E1500
Tumour size	0.18	0.0009	0.18	0.0009
Age at diagnosis	−0.11	0.051	−0.10	0.060
ER	−0.26	< 0.0001	−0.24	< 0.0001
PgR	−0.37	< 0.0001	−0.31	< 0.0001
Ki 67	0.48	< 0.0001	0.49	< 0.0001
Elston	0.58	< 0.0001	0.57	< 0.0001
Mitotic count	0.56	< 0.0001	0.55	< 0.0001
Tubuli	0.25	< 0.0001	0.21	< 0.0001
Nuclear atypia	0.49	< 0.0001	0.50	< 0.0001
HER 2	0.24	< 0.0001	0.19	< 0.0003
Cyclin A	0.57	< 0.0001	0.59	< 0.0001
Cyclin B	0.61	< 0.0001	0.62	< 0.0001

r, Spearman's rank correlation test coefficient.

Figure 1. Flow chart diagram cyclin E.

Figure 2. The relationship between a high or low level of Cyclin E1 expression and the period free from distant metastasis in the patients who died from their breast cancer.

Table III. Univariate and multivariate analysis of prognostic indicators of death from low- risk breast cancer.

Univariate analysis					Mutivariate analysis
	Reference	OR	95% CI	p-Value	OR	95% CI	p-Value
Age at diagnosis	< 70 year	2.7	1.4–3.6	0.0005	2.4	1.4–4.1	0.002
Tumour size	≤ 20 mm	2.2	1.4–3.5	0.0005	1.7	1.0–2.9	0.06
ER	> 10%	2.8	1.7–4.6	0.00003	1.9	1.1–3.3	0.02
PR	> 10%	2.7	1.8–4.2	0.000006
Elston	1–2 grad	3.1	1.8–5.1	0.00002
Ki67 max	≤ 22%	1.7	1.1–2.7	0.01
HER2status	negative	1.0	0.4–2.3	1.0
Cyclin A	≤ 11%	3.4	2.1–5.5	0.000001
Cyclin B	≤ 6%	2.9	1.8–4.6	0.000007
Cyclin E 200	≤ 20.45%	2.3	1.5–3.6	0.0003	2.1	1.2–3.5	0.005
Cyclin E 500	≤ 17.85%	2.3	1.5–3.7	0.0002

CI, confidence interval; OR, odds ratio.