Advanced search
Publication Cover
Acta Oncologica Volume 55, 2016 - Issue 2
Journal homepage
1,278
Views
7
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLE: Lung Cancer

Keratin 34betaE12/keratin7 expression is a prognostic factor of cancer-specific and overall survival in patients with early stage non-small cell lung cancer

Mette PøhlDepartment of Oncology, Odense University Hospital, Odense, Denmark;Institute of Clinical Research, University of Southern Denmark, Odense, Denmark;Department of Oncology, Rigshospitalet, Copenhagen, DenmarkCorrespondence[email protected]
,
Karen Ege OlsenInstitute of Clinical Research, University of Southern Denmark, Odense, Denmark;Department of Pathology, Odense University Hospital, Odense, Denmark
,
Rene HolstDepartment of Statistics, University of Southern Denmark, Odense, Denmark
,
Tom DonnemInstitute of Clinical Medicine, University of Tromso, Tromso, Norway;Department of Oncology, University Hospital of North Norway, Tromso, Norway
,
Lill-Tove BusundInstitute of Medical Biology, University of Tromso, Tromso, Norway;Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway
,
Roy M. BremnesInstitute of Clinical Medicine, University of Tromso, Tromso, Norway;Department of Oncology, University Hospital of North Norway, Tromso, Norway
,
Samer Al-SaadInstitute of Medical Biology, University of Tromso, Tromso, Norway;Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway
,
Sigve AndersenInstitute of Clinical Medicine, University of Tromso, Tromso, Norway;Department of Oncology, University Hospital of North Norway, Tromso, Norway
,
Elin RichardsenInstitute of Medical Biology, University of Tromso, Tromso, Norway;Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway
,
Henrik J. DitzelDepartment of Oncology, Odense University Hospital, Odense, Denmark;Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark
&
Olfred HansenDepartment of Oncology, Odense University Hospital, Odense, Denmark;Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
 show all
Pages 167-177 | Received 15 Jan 2015, Accepted 29 Apr 2015, Published online: 09 Jun 2015

Figures & data

Figure 1. Immunohistochemical analysis of keratin expression pattern in non-small cell lung cancer (NSCLC); different cytoplasmic staining scores for tumor cell are shown. For K7, cores with tumor cell content of ≥ 10% staining were considered positive and tumors with ≥ 1% staining for 34βE12 were considered positive. (A) Tumor cells positive for 34βE12 and negative for K7 (34βE12+/K7+). (B) Tumor cells positive for 34βE12 and for K7 (34βE12+/K7+). (C) Tumor cells negative for 34βE12 and positive for K7 (34βE12-/K7+). (magnification 10×).
Figure 1. Immunohistochemical analysis of keratin expression pattern in non-small cell lung cancer (NSCLC); different cytoplasmic staining scores for tumor cell are shown. For K7, cores with tumor cell content of ≥ 10% staining were considered positive and tumors with ≥ 1% staining for 34βE12 were considered positive. (A) Tumor cells positive for 34βE12 and negative for K7 (34βE12+/K7+). (B) Tumor cells positive for 34βE12 and for K7 (34βE12+/K7+). (C) Tumor cells negative for 34βE12 and positive for K7 (34βE12-/K7+). (magnification 10×).

Table I. Patient demographics and prognostic covariates and cancer-specific survival by univariate log-rank test.

Figure 2. Cancer-specific survival related to keratin expression patterns using log-rank test. (A) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the Danish cohort (n = 177). (B) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the Norwegian validation cohort (n = 284). (C) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the combined Danish and Norwegian cohorts (n = 461).
Figure 2. Cancer-specific survival related to keratin expression patterns using log-rank test. (A) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the Danish cohort (n = 177). (B) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the Norwegian validation cohort (n = 284). (C) Kaplan-Meier cancer-specific survival curves according to keratin expression patterns (34βE12/K7) in the combined Danish and Norwegian cohorts (n = 461).
Figure 3. Cancer-specific survival related to the significant interaction between lymph node status and keratin expression in the Danish and Norwegian validation cohorts. The interaction between keratin expression and lymph node status demonstrated that the effect of keratin expression is most significant in lymph node-positive tumors. (A-B) Cancer-specific survival and keratin expression in (A) lymph node-negative and (B) lymph node-positive tumors in the Danish cohort. (C-D) Cancer-specific survival and keratin expression in (C) lymph node-negative tumors and (D) lymph node-positive tumors in the Norwegian validation cohort.
Figure 3. Cancer-specific survival related to the significant interaction between lymph node status and keratin expression in the Danish and Norwegian validation cohorts. The interaction between keratin expression and lymph node status demonstrated that the effect of keratin expression is most significant in lymph node-positive tumors. (A-B) Cancer-specific survival and keratin expression in (A) lymph node-negative and (B) lymph node-positive tumors in the Danish cohort. (C-D) Cancer-specific survival and keratin expression in (C) lymph node-negative tumors and (D) lymph node-positive tumors in the Norwegian validation cohort.

Table II. Results of multiple cox regression analysis and association with significant prognostic factors and cancer-specific survival.

Figure 4. Cancer-specific and overall survival related to the significant interaction between lymph node status and keratin expression in the combined Danish-Norwegian cohort. The interaction between keratin expression and lymph node status demonstrated that the effect of keratin expression is mainly significant in lymph node-positive tumors. (A-B) Cancer-specific survival and keratin expression in (A) lymph node-negative tumors and (B) lymph node-positive tumors in the combined cohort. (C-D) Overall survival and keratin expression in (C) lymph node-negative tumors and (D) lymph node-positive tumors in the combined cohort.
Figure 4. Cancer-specific and overall survival related to the significant interaction between lymph node status and keratin expression in the combined Danish-Norwegian cohort. The interaction between keratin expression and lymph node status demonstrated that the effect of keratin expression is mainly significant in lymph node-positive tumors. (A-B) Cancer-specific survival and keratin expression in (A) lymph node-negative tumors and (B) lymph node-positive tumors in the combined cohort. (C-D) Overall survival and keratin expression in (C) lymph node-negative tumors and (D) lymph node-positive tumors in the combined cohort.
Supplemental material

ionc_a_1049291_sm0725.pdf

Download PDF (1.5 MB)

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.