Inhibition of hyaluronan synthesis in rats reduces renal ability to excrete fluid and electrolytes during acute hydration

Figures & data

Figure 1. Hyaluronan (HA) content in cortex and inner medulla (papilla) in control rats and in rats treated with the hyaluronan synthesis inhibitor 4-MU. *p < 0.05 versus control.

Table I. Glomerular filtration rate (GFR), mean arterial blood pressure (MAP), urine flow rate, and sodium excretion in control rats (C) and in rats treated with the hyaluronan synthesis inhibitor 4-MU. Each period corresponds to 45 min in consecutive order, p < 0.05 versus Control period of same group. Values are means ± SEM.

	Control period	Hydration 1	Hydration 2	Hydration 3
GFR (mL/min/g kw)
C	0.90 ± 0.27	0.78 ± 0.22	0.93 ± 0.16	0.90 ± 0.10
4-MU	0.84 ± 0.12	0.65 ± 0.12	0.80 ± 0.15	0.82 ± 0.15
MAP (mmHg)
C	109 ± 11	104 ± 11	108 ± 9	105 ± 8
4-MU	97 ± 4	96 ± 4	96 ± 3	92 ± 3
Urine flow rate (µL/min/g kw)
C	1.31 ± 0.45	1.59 ± 0.32	2.61 ± 0.89	2.54 ± 0.49 (p < 0.05)
4-MU	0.82 ± 0.13	1.16 ± 0.34	1.48 ± 0.41	1.52 ± 0.30 (p < 0.05)
Sodium excretion (µL/min/g kw)
C	0.05 ± 0.01	0.07 ± 0.02	0.08 ± 0.02	0.07 ± 0.05
4-MU	0.07 ± 0.02	0.06 ± 0.02	0.06 ± 0.02	0.07 ± 0.02

kw = kidney weight.

Figure 2. Accumulated urine excretion during hydration in control rats and in rats treated with the hyaluronan synthesis inhibitor 4-MU. *p < 0.05 versus control. (kw = kidney weight).

Figure 3. Accumulated osmolar excretion during hydration in control rats and in rats treated with the hyaluronan synthesis inhibitor 4-MU. *p < 0.05 versus control. (kw = kidney weight).