Figures & data
Figure 1. MMP-8 levels analyzed by IFMA in effusions of children with otitis media (n = 70), of which 39 were classified to mucoid and 31 to serous effusions. Median MMP-8 levels were significantly higher in mucoid middle ear fluid (MEF) than in serous MEF, analyzed by Mann-Whitney test.
Figure 2. Representative Western immunoblot analysis of MMP-8 (collagenase-2) in middle ear effusions. Lanes 2–4 represent mucous and lanes 5–7 serous middle ear effusions. Lane 8 represents human neutrophil (PMN) and lane 9 human rheumatoid synovial fibroblast (F) culture media fluid used as positive controls for pro- and active PMN and mesenchymal (Mes)-type MMP-8 isoforms, respectively, of which mobilities are indicated on the right. Mobilities of the molecular weight markers (MW) are indicated on the left (lane 1).
Figure 3. Representative gelatin-zymography analysis of molecular forms and degree of activation of gelatinases (MMP-2 and -9) in middle ear effusion. Lanes 2–4 represent mucous and lanes 5–7 serous middle ear effusion. Lane 8 represents human 92 kDa MMP-9 and lane 9 represents human 72 kDa MMP-2. Mobilities of 92 kDa proMMP-9, 82 kDa active MMP-9, 72 kDa proMMP-2, and active 62 kDa MMP-2, as well as 120 kDa gelatinase (MMP-9-NGAL) complex, are indicated on the right. Mobilities of the molecular weight markers (MW) are indicated on the left (lane 1).
Table I. Gelatinolytic activity in middle ear fluid (n = 70) of children with early phase of otitis media with effusion (OME). Gelatinolytic activity was assayed by gelatin-zymography.