The antioxidant role of oral administration of garlic oil on renal ischemia–reperfusion injury

Figures & data

TABLE 1.  Serum clinical parameters in control, sham, I/R, and I/R + GO rats

Group	n	Urea (mg/dL)	Creatinine (mg/dL)	Cystatin C (mg/dL)
Control	10	14.1 ± 0.75	0.5 ± 0.1	0.13 ± 0.03
Sham	10	15 ± 2.4	0.61 ± 0.05	0.18 ± 0.02
I/R	10	99.8 ± 15.3	2.18 ± 1.5	0.29 ± 0.04
I/R + GO	10	69.2 ± 17.3	1.61 ± 0.3	0.22 ± 0.05
p-Value		<0.01	<0.01	<0.01

Notes: I/R, ischemia/reperfusion; I/R + GO, ischemia/reperfusion + garlic oil.

*p < 0.01 compared to control, sham, and I/R + GO.

TABLE 2.  Oxidative and antioxidative parameters of the tissues

	Control	Sham	I/R	I/R + GO	p-Value
TAC	4.45 ± 2.6	4.27 ± 0.88	3.06 ± 0.70	4.45 ± 0.64	<0.01
TOS	8.80 ± 1.11	9.0 ± 1.34	12.9 ± 1.60	10.8 ± 1.89	<0.01
OSI	1.97 ± 0.61	2.19 ± 0.59	4.58 ± 1.76	2.49 ± 0.73	<0.01
CAT	20.8 ± 1.90	19.8 ± 2.12	10.7 ± 1.98	18.4 ± 2.18	<0.01
MPO	10.03 ± 0.92	11.0 ± 1.13	14.4 ± 1.69	10.9 ± 1.29	<0.01
NO	0.15 ± 0.02	0.18 ± 0.03	0.29 ± 0.05	0.24 ± 0.06	<0.01
PC	0.50 ± 0.56	1.12 ± 0.55	2.01 ± 0.2	1.76 ± 0.4	<0.01

Notes: I/R, ischemia/reperfusion; I/R + GO, ischemia/reperfusion + garlic oil; TAC, total antioxidant capacity, nmol H₂O₂ Equiv./mg protein; TOS, total oxidant status, nmol H₂O₂ Equiv./mg protein; OSI, oxidative stress index, arbitrary unit; CAT, catalase, MU/L; MPO, myeloperoxidase activity, mU/g protein; NO, nitrite oxide, nmol/g wet tissue; PC, protein carbonyl, nmol/mg protein.

*p < 0.01 compared to control, sham, and I/R + GO.

FIGURE 1.  Normal renal tissue with no pathological change from control group.

FIGURE 2.  Tubular damage characterized by loss of brush border, lumen dilatation or collapse, and cellular detachment from tubular basement membranes were observed (I/R group).

FIGURE 3.  Lesions were milder (garlic oil treatment group).