apoE Expression in Glomerulus and Correlation with Glomerulosclerosis Induced by Adriamycin in Rats

Figures & data

Table 1. Parameters in two groups (mean ± SD)

Parameters	9 weeks		13 weeks
	SHO	GS	SHO	GS
BUN (mmol/L)	7.83 ± 1.87	13.11 ± 2.76	8.10 ± 1.65	16.39 ± 3.37
Scr (µmol/L)	30.67 ± 5.13	43.17 ± 7.96	31.33 ± 4.86	49.83 ± 8.38
24UTP (mg/24 h)	16.42 ± 2.13	50.24 ± 6.32	17.59 ± 3.28	65.09 ± 7.64
GSI (%)	1.25 ± 0.21	17.64 ± 3.52	1.36 ± 0.27	23.63 ± 4.63
Col-IV (%)	17.52 ± 6.31	40.08 ± 8.32	18.24 ± 6.42	56.27 ± 8.53
FN (%)	7.26 ± 1.47	15.34 ± 4.53	7.73 ± 1.33	23.87 ± 7.63
α-SMA (%)	0.56 ± 0.13	23.42 ± 5.42	0.56 ± 0.28	34.32 ± 6.03
TGF-β1 (%)	0.46 ± 0.29	21.36 ± 4.84	0.51 ± 0.31	30.25 ± 5.78

Notes: SHO, sham operation group; GS, glomerulosclerosis model group; BUN, blood urea nitrogen; Scr, serum creatinine; 24UTP, 24-h urine protein excretion; GSI, glomerulosclerosis index; Col-IV, collagen IV; FN, fibronectin; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor-β1.

*p < 0.01 compared with SHO.

Figure 1. Glomerular morphology was normal in sham group (A₁: 9 weeks; A₂: 13 weeks; HE). Proliferation occurring in the majority of glomerular mesangial cells and extracellular matrix in GS group, and degeneration of glomerular epithelial cells and infiltration of widespread mononuclear cells were shown (A₃: 9 weeks; A₄: 13 weeks; HE), especially in 13 weeks of GS group (A₄). Representative samples of immunohistochemical staining for glomerular Col-IV (SHO: B₁: 9 weeks, B₂: 13 weeks; GS: B₃: 9 weeks and B₄: 13 weeks), FN (SHO: C₁: 9 weeks, C₂: 13 weeks; GS: C₃: 9 weeks and C₄: 13 weeks), α-SMA (SHO: D₁: 9 weeks, D₂: 13 weeks; GS: D₃: 9 weeks and D₄: 13 weeks), TGF-β1 (SHO: E₁: 9 weeks, E₂: 13 weeks; GS: E₃: 9 weeks and E₄: 13 weeks), and apoE (SHO: F₁: 9 weeks, F₂: 13 weeks; GS: F₃: 9 weeks and F₄: 13 weeks) were observed in all groups. Sham group (B₁, B₂, C₁, C₂, D₁, D₂, E₁, E₂, F₁, and F₂): positive staining (in brown) was faint in glomerulus, glomerular endothelial cells, glomerular basement membrane, mesangial cells, and visceral epithelial cells. GS group (B₃, B₄, C₃, C₄, D₃, D₄, E₃, E₄, F₃, and F₄): positive staining was strong in most glomerulus, glomerular endothelial cells, glomerular basement membrane, mesangial cells, and visceral epithelial cells, especially in those of 13 weeks (B₄, C₄, D₄, E₄, and F₄). Magnification 400×.

Notes: SHO, sham operation group; GS, glomerulosclerosis model group; HE, hematoxylin and eosin; FN, fibronectin; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor-β1; apoE, apolipoprotein E.

Table 2. Protein expression of apoE in glomerulus and mRNA expression of apoE in renal tissue of the two groups (mean ± SD)

Groups	n	apoE protein		9 weeks		13 weeks
		9 weeks	13 weeks	ΔCT	Fold	ΔCT	Fold
SHO	20	17.69 ± 5.63	18.91 ± 5.43	3.43 ± 0.50	1	3.40 ± 0.49	1.02
GS	20	28.47 ± 6.42	34.17 ± 8.28	1.20 ± 0.10	4.69	0.84 ± 0.09	6.02

Notes: SHO, sham operation group; GS, glomerulosclerosis model group.

*p < 0.01 compared with SHO.