Effects of Phosphonoformic Acid and Renagel on Renal Type IIa Sodium-Dependent Phosphate Cotransporter mRNA Expression in Hyperphosphatemia Rats

Figures & data

Table 1. Changes in Scr and Ccr in Nx and sham-operated rats.

		Preoperative		Postoperative
Group	n	Scr (mol/L)	Ccr (mL/min)	Scr (mol/L)	Ccr (mL/min)
Sham	12	87.99 ± 9.57	0.85 ± 0.07	102.01 ± 17.30	0.73 ± 0.08
Nx	30	82.71 ± 9.53	0.82 ± 0.08	147.30 ± 18.45	0.48 ± 0.08

Notes: All results were from three independent experiments, and analysis was done by a core service center in a double-blinded manner. Values are mean ± SD. Scr, serum creatinine; Ccr, creatinine clearance rate.

*Denotes p < 0.05 versus preoperative values.

Table 2. Comparison of serum P, iCa, and iPTH in different rat groups.

	Day 2			Day 7			Day 14
										1,25(OH)2D3
Group	P (mmol/L)	iCa (mmol/L)	iPTH (pg/mL)	P (mmol/L)	iCa (mmol/L)	iPTH (pg/mL)	P (mmol/L)	iCa (mmol/L)	iPTH (pg/mL)	(pg/mL)
Nx + HP	4.04 ± 0.58	1.19 ± 0.05	68.53 ± 31.23^**	4.09 ± 0.57	1.21 ± 0.20	72.33 ± 16.64^**	3.93 ± 0.27	1.15 ± 0.08	75.50 ± 20.19^**	79.77 ± 1.85
Nx + LP	2.38 ± 0.55	1.25 ± 0.11	31.85 ± 18.51	2.40 ± 0.31	1.34 ± 0.07	37.13 ± 14.09	2.42 ± 0.84	1.20 ± 0.08	30.55 ± 2.33	77.51 ± 3.63
HP + PFA	3.95 ± 0.29	1.19 ± 0.04	45.27 ± 15.86	3.86 ± 1.08	1.22 ± 0.09	61.84 ± 35.60	3.84 ± 1.04	1.10 ± 0.08	41.73 ± 20.19^***	76.21 ± 4.32
HP + Saline	3.69 ± 0.31	1.16 ± 0.06	67.46 ± 24.32	4.07 ± 0.48	1.19 ± 0.18	70.16 ± 20.15	4.05 ± 0.32	1.17 ± 0.09	71.09 ± 19.36	78.34 ± 2.15
HP + Renagel	3.98 ± 0.33	1.16 ± 0.05	33.33 ± 9.82	3.49 ± 0.10^****	1.22 ± 0.04	35.72 ± 7.78^*****	3.23 ± 0.57^****	1.18 ± 0.14	33.63 ± 5.16^*****	78.6 ± 2.87
Sham + HP	3.00 ± 0.41	1.20 ± 0.05	30.20 ± 19.46	3.25 ± 0.37	1.30 ± 0.09	34.08 ± 2.94	3.07 ± 0.64	1.26 ± 0.04	32.98 ± 5.37	82.18 ± 3.07
Sham + LP	2.70 ± 0.49	1.22 ± 0.09	33.99 ± 18.58	3.06 ± 0.74	1.29 ± 0.09	33.08 ± 11.18	3.05 ± 0.84	1.14 ± 0.11	30.72 ± 5.48	82.29 ± 2.37

Notes: All results were from three independent experiments, and analysis was done by a core service center in a double-blinded manner. Values are mean ± SD.

*Significantly different from Nx + LP and sham-operated groups at p < 0.05, ^**significantly different from Nx + LP and sham-operated groups at p < 0.05, ^***significantly different from Nx + HP and HP + Saline groups at p < 0.05, ^****significantly different from Nx + HP group at p < 0.05, and ^*****significantly different from Nx + HP group at p < 0.05.

Figure 1. Comparison of calcium–phosphorus product in HP + Renagel versus Nx + HP rats.

Notes: All results were from three independent experiments, and analysis was done by a core service center in a double-blinded manner. Data are presented as mean ± SD. *Denotes p < 0.05 versus Nx + HP group.

Figure 2. Urinary FEP in different rat groups.

Notes: All results were from three independent experiments, and analysis was done by a core service center in a double-blinded manner. Data are presented as mean ± SD. *Significantly different from Sham + HP group at p < 0.05, ^**significantly different from Nx + HP group at p < 0.05, and ^***significantly different from Nx + HP group at p < 0.05.

Figure 3. NaPi-2 mRNA expression in different rat groups. (A) Expression of NaPi-2 mRNA by RT-PCR. PCR products were separated in 1% agarose gel and stained with ethidium bromide. (B) Densitometric data of renal NaPi-2 mRNA abundance. NaPi-2 mRNA signals were quantified by image analysis software BandScan 4.3. Data were expressed versus GAPDH and compared with ANOVA.

Notes: All results were from three independent experiments, and analysis was done by a core service center in a double-blinded manner. *Significantly different from Sham + LP group at p < 0.05, ^**significantly different from Nx + LP group at p < 0.01, and ^***significantly different from Nx + HP group at p < 0.01.