Nec-1 Protects against Nonapoptotic Cell Death in Cisplatin-Induced Kidney Injury

Figures & data

Figure 1. Representative fluorescence microscopy images of human proximal tubule cells (HK-2): (A) Control group, (B) Cis group, (C) Nec-1 + Cis group, (D) Cis + z-VAD group, and (E) Nec-1 + Cis + z-VAD group.

Figure 2. Protective effects of inhibitors [necrostatin-1 (Nec-1) and benzyloxy-carbonyl-Val-Ala-Asp-fluoromethyl ketone (z-VAD-fmk)] on apoptosis induced by cisplatin in human proximal tubule cells (HK-2).Note: *p < 0.05, Cis group and Nec-1 + Cis group versus Control group, ^#p < 0.05, Cis group and Nec-1 + Cis group versus Nec-1 + Cis + z-VAD group.

Figure 3. The effects of inhibitors [necrostatin-1 (Nec-1) and benzyloxy-carbonyl-Val-Ala-Asp-fluoromethyl ketone (z-VAD-fmk)] on cell viability in human proximal tubule cells (HK-2) treated with cisplatin.Note: *p < 0.05, Cis group, Nec-1 + Cis group and Cis + z-VAD group versus Control group, ^#p < 0.05, Cis group, Nec-1 + Cis group and Cis + z-VAD group versus Nec-1 + Cis + z-VAD group.

Figure 4. Flow cytometry analysis of apoptosis induced by cisplatin in human proximal tubule cells (HK-2): (A) Control group, (B) Cis group, (C) Nec-1 + Cis group, (D) Cis + z-VAD group, and (E) Nec-1 + Cis + z-VAD group.

Note: PI, propidium iodide.

Table 1. Morphological features of necrosis and apoptosis.

Cell death	Characteristics	Reference(s)
Necrosis	Accidental and uncontrolled/massive tissue damage	^12,4
	Membrane and organelle disruption/cell swelling/mitochondria impairment/cell lyses/inflammatory response	^13
Apoptosis	Controlled cell death/energy dependent	^14,15
	Cytoplasm and chromatin condensation/DNA degradation/nuclear fragmentation/cell shrinkage/plasma membrane blebbing/formation of apoptotic bodies	^16

Teng X, Degterev A, Jagtap P, . Structure-activity relationship study of novel necroptosis inhibitors. Bioorg Med Chem Lett. 2005;15(22):5039–5044.

 PubMed Web of Science ®Google Scholar

Galluzzi L, Kroemer G. Necroptosis: A specialized pathway of programmed necrosis. Cell 2008;135(7):1161–1163.

 PubMed Web of Science ®Google Scholar